摘要
目的 探讨粘附分子P-选择素与树突状细胞(DC)在大鼠单侧输尿管梗阻后肾组织中的表达和分布,以及抗P-选择素单抗的抗粘附调抑作用。方法 建立大鼠单侧输尿管梗阻(UUO)模型,随机将大鼠分为手术组和抗P-选择素单抗治疗组各18只,按不同梗阻时间(1、3、7d)再分为3组,另设假手术组(n=6)作为对照。分别采用免疫组化LSAB法和免疫双染与荧光图像分析法,观察P-选择素及DC在肾组织中表达和分布变化。结果 大鼠UUO第1d起即见P-选择素以肾小管上皮细胞为主在肾小管间质中表达上调,伴随CD1a^+CD80^+DC在以肾间质为主部位的浸润;第3 d P-选择素明显表达,且CD1a^+CD80^+DC显著增多;至第7 d P-选择素表达遂下调,而CD1a^+CD80^+DC在肾间质分布聚集达峰,出现了明显的肾小管间质病理损伤经抗P-选择素单抗处理后,大鼠肾组织P-选择素表达受到抑制,CD1a^+CD80^+DC分布聚集减少,且肾小管间质病理损伤减轻。结论 提示树突状细胞参与了肾小管间质纤维化早期病理损伤过程,其肾组织迁移聚集可能与P-选择素早期介导有关,抗P-选择素单抗对此具有抗粘附调抑和肾脏保护作用。
Objective To explore the expression of P- selectin and the distribution of dendritic cells( DCs) in rat kidney with unilateral ureteral obstruction, as well as the preventive effect of anti-P-selectin lectin-EGF domain monoclonal antibody ( PsL-EGFmAb). Methods Rat models of unilateral ureteral obstruction were established, which were divided into sham group( n =6 ) , untreated group( n = 18 ) and treated group with PsL-EGFmAb( n = 18) . DCs were analyzed with axioplan 2 microscopy, while P-selectin was analysed by immunohistochemistry LSAB, the alter of renal pathology was observed at the same time. Results P-selectin was presented in renal tubular epithelial cell after unilateral ureteral obstruction on day 1 , and CD1a+ CD80 + DC was also found in renal interstitium; P-selectin and DCS presented was increased evidently on the 3rd day; The expression of P-selectin was decreased, but the accumulation of DCs were the most in renal interstitium on the 7' day. These changes became less conspicuous in rat kidney treated with PsL-EGFmAb. Conclusion DCs immigration and accumulation may play an important role in early tu-bulointerstitial lesions. PsL-EGFmAb has anti-adhesive effect on it.
出处
《上海第二医科大学学报》
CSCD
2004年第3期174-177,共4页
Acta Universitatis Medicinalis Secondae Shanghai
基金
国家自然科学基金(39970340)
上海市自然科学基金(02ZB14041.034119916)
