摘要
目的探讨粘附分子Ｐ选择素与人类肾小球肾炎的关系。方法采用免疫组化（ＬＳＡＢ）和原位杂交方法观察１３３例肾小球疾病患者肾组织中Ｐ选择素表达情况。根据肾小球内炎细胞浸润、细胞增生及硬化程度分为三组。Ⅰ组：肾小球内无明显炎细胞浸润；Ⅱ组：肾小球有较明显炎症病变和细胞增生；Ⅲ组：肾小球炎症过程趋向静止及（或）出现较多肾小球硬化。结果正常肾组织中Ｐ选择素基本不表达。在肾小球疾病肾组织中，Ｐ选择素在肾小管上皮细胞表达显著高于肾小球和肾间质；在肾小球表达以血小板表面为主，其中Ⅱ组肾小球Ｐ选择素表达水平与Ⅰ组（Ｐ＜０．０５）、Ⅲ组（Ｐ＜０．０１）比较有显著性差异。肾小管间质病变程度与肾小管上皮细胞、间质表达Ｐ选择素显著相关（ｒｓ＝０．３９５和ｒｓ＝０．３３７，Ｐ＜０．０１）。原位杂交发现肾小球、小管上皮细胞、间质、血管内皮部位均有Ｐ选择素ｍＲＮＡ表达，与蛋白质表达部位一致。结论在人类增生性肾小球肾炎发病早期，Ｐ选择素介导了肾内血小板聚集活化、炎细胞浸润。
Objective To examine the relationship between Pselectin expression and human glomerulonephritisMethods Pselectin expression in renal biopsies of glomerulonephritis patients (n=133) was investigated by immunohistochemical and in situ hybridization analysesThey were divided into 3 groups based on the degree of neutrophil infiltration and cells proliferation or sclerosisResults In glomerulonephritis,the upregulated Pselectin expression in tubular epithelium was significantly higher than in glomeruli and interstitiumThere were strong correlations between the degree of tubulointerstitial lesions and the expression of Pselectin on tubular epithelium or within interstitiumFurthermore,Pselectin mRNA signals were also detected in the cytoplasma of glomerulus,tubular epithelial cells,intertitium and vascular endotheliumConclusions Pselectin might mediate intraglomerular platelets aggregation,activation and leukocyte accumulation in the early stages of human proliferative glomerulonephritisThe upregulation of Pselectin in interstitium was associated with interstitial fibrosis and tubular atrophy and may contribute to the progression of glomerulonephritis
出处
《中华肾脏病杂志》
CAS
CSCD
北大核心
1998年第3期155-158,共4页
Chinese Journal of Nephrology
基金
卫生部科研基金
关键词
粘附分子
P选择素
肾小球肾炎
Adhesion molecules Pselectin Glomerulonephritis
