摘要
用SDS聚丙烯酰胺凝胶电泳,分析了来自小麦(Triticum aestivum L.)纯合花粉植株的幼穗愈伤组织的9个无性系及其供体植株系的种子的醇溶蛋白和谷蛋白。发现有6个无性系的醇溶蛋白和谷蛋白的电泳谱带与供体植株完全相同,另3个无性系的两种蛋白的谱带彼此相同,但与供体株系有明显差别。这表明在无性系诱导的组织培养过程中,发生了基因水平上的体细胞无性系变异。
Gliadin and glutenin of seeds of a pollen-derived pure line 'H-33' and its 9 stable somac-lones of wheat (Triticutn aestivum L.) from inflorescence callus were investigated using SDS-PAGE. Among the 9-somaclones 6 were identical to the 'H-33' in SDS-PAGE patterns of of gliadin and glutenin. The other 3 somaclones were identical with each other, but different from 'H-33'. The varied electrophoretic bands of seed protein showed that somaclonal variation had occured at gene level during tissue culture in which the somaclones were produced.
基金
国家重点科技(攻关)计划资助项目
关键词
小麦
醇溶蛋白
体细胞
无性系
Somaclonal variation
Triticum aestivum
Gliadin
Glutenin
SDS-PAGE
