摘要
目的 探讨CyclinE在肝细胞癌中的表达及其意义。方法 应用同一组织连续切片免疫组化SABC法 ,检测CyclinE在 45例肝细胞癌 (HCC)及 3 0例癌旁肝组织中的表达及其与临床病理资料的关系。其中门静脉癌栓形成者 12例 ,有转移者 11例 ,肝内肿瘤播散或有卫星结节者 12例 ,肿瘤包膜侵袭者 19例 ,以上 4项中有任何 1项阳性或 1项以上阳性者归入侵袭转移组。结果 CyclinE在HCC组织中阳性表达率 3 5 .6% (16/ 45 ) ,癌旁肝组织无阳性表达。CyclinE表达与年龄、性别、乙肝表面抗原、血清甲胎蛋白、肿瘤大小、包膜形成、肝外转移及癌灶数目无关 (P >0 .0 5 ) ,与组织分化 ,癌栓形成有关 (P <0 .0 5 )。CyclinE在侵袭转移组较非侵袭转移组表达增高 (P <0 .0 5 )。.结论 CyclinE与肝细胞癌的发生 ,分化及侵袭转移有关 ,检测其表达有助于协助临床诊断治疗及判断预后。
Objective To study the significance of expression of Cyclin E in hepatocellular carcinoma (HCC). Methods Cyclin E expressions were examined in 45 specimens of HCC and 30 specimens of paracancer tissues, and the relationship between Cyclin E with the clinico-pathological parameters was determined.Among the 45 patients ,19 were poor encapsulatic; 12 patients had portal vein thrombus; 11 had extrahepatic metastasis;12 had intrahepatic metastasis.Any patient had one or more of 4 conditions mentioned above was considered as invasion and metastasis. Results The overexpression of Cyclin E in HCC tissues was seen in 16 cases (35.6%) ,but not seen in paracancer tissues. Cyclin E overexpression occurred more frequently in HCC patients with poor differentiation and portal vein thrombus ( P <0.05). The overexpression of Cyclin E in HCC patients with invasive and metastsis was significantly higher than that in non-invasive and non-metastasis patients ( P <0.05). There were no significant difference between the expression of Cyclin E with patients′ age, sex, tumor size, HBsAg,AFP,capsure formation and tumor number( P >0.05). Conclusions Expression of Cyclin E is related with HCC carcinogenesis; differentiation , invasion and metastasis, detection of expression of Cyclin E may be helpful in the diagnosis, treatment and prediction of the prognosis of patients with HCC.
出处
《中国普通外科杂志》
CAS
CSCD
2004年第3期193-195,共3页
China Journal of General Surgery
