摘要
目的:探讨LRP、GST-π在肝细胞癌中的表达及其与临床病理特征的关系。方法:应用RT-PCR和免疫组织化学方法检测LRP和GST-πmRNA及蛋白在34例的肝细胞癌、19例正常肝组织中的表达。结果:LRP和GST-πmRNA的表达在肝癌组为0.53±0.28、0.75±0.19,正常肝组织组为0.22±0.10、0.26±0.12,差异有统计学意义(P<0.05)。免疫组化结果显示,LRP和GST-π的阳性表达率在肝癌组为55.88%、67.65%,在正常肝组织组为21.05%、31.58%,差异有统计学意义(P<0.05)。LRP、GST-πmRNA和蛋白在肝癌组的表达高于正常肝组织组,且低分化(Ⅲ、Ⅳ级)肝癌组织的表达高于高分化者(Ⅰ、Ⅱ级)(P<0.05),LRP与GST-π之间的表达无线性相关。结论:在肝细胞癌中存在原发性多药耐药现象,且多种耐药机制并存。LRP、GST-π在肝细胞癌中高表达,且随着肿瘤分化程度的增高而降低。
Objective：To investigate the expressions of LRP and GST - π in hepatocellular carcinoma and its relationship with pathologic features. Methods：The expressions of LRP and GST - π in hepatocellular carcinoma were examined by RT - PCR and immunohistochemistry stain in 34 cases of hepatocellular carcinoma and 19 cases of normal hepatic tissues. Results：The expression of LRP and GST - π mRNA were 0.53 ±0.28,0.75 ±0.19 in hepatocellular carcinoma and 0.22 ± 0.10,0.26 ± 0.12 in normal hepatic tissues （ P 〈 0. 05 ）. The positive rates of LRP and GST - π were 55.88% ,67.65% in hepatocellular carcinoma and 21.05% ,31.58% in normal hepatic tissues（P 〈 0.05）, respectively. The expressions of LRP and GST- π in hepatocellular carcinoma were higher than those in normal tissues. And the expressions in poorly differentiated hepatocellular carcinoma were higher than those in well - differentiated tissues（P 〈0.05）. The expressions of LRP and GST-π had no linear correlation with each other. Conclusion：Primary multidrug resistance was exsisted in hepatocellular carcinoma and there were many mechanisms of muhidrug resistance. LRP and GST -π were highly expressed in hepatocellular carcinoma, the higher differentiation of hepatocellular carcinoma,the lower expression was.
出处
《现代肿瘤医学》
CAS
2010年第1期102-105,共4页
Journal of Modern Oncology
基金
山东省"泰山学者"建设工程专项经费
山东省科技攻关计划项目(编号:2006GG3202016)
关键词
多药耐药
肝细胞癌
RT—PCR
免疫组织化学染色
multidrug resistance
hepatocellular carcinoma
RT - PCR
immunohistochemistry stain
