摘要
AIM: To investigate the anti-cancer effect and the immunological mechanism of ultrasound-guided intratumoral injection of Chinese medicine "Star-99" in hepatocellular carcinoma (HCC) of nude mice.METHODS: Twenty-eight human hepatocellular carcinoma SMMC-7721 transplanted nude mice, 14 of hypodermically implanted and 14 of orthotopic liver transplanted, were randomly divided into three groups of which 14 mice with Star-99, and 7 with ethanol and saline respectively. Ten days after the transplantation the medicines were injected into the tumors of all the nude mice once every 5 days.After 4 injections the nude mice were killed. The diameters of three dimension of the tumors were measured by high frequency ultrasound before and after the treatment and the tumor growth indexes* (TGI) were calculated.Radioimmunoassay was used to detect the serum levels of interleukin-2 (IL-2) and tumor necrosis factor (TNF)-alpha.The tumor tissues were sent for flow cytometry (FCM) DNA analysis. Apoptotic cells were visualized by TUNEL assay.All the experiments were carried out by double blind method. zRESULTS: The TGI of Star-99 group (0.076±0.024) was markedly lower than that of the saline group (4.654±1.283)(P＜0.01). It also seemed to be lower than that of the ethanol group (0.082±0.028), but not significantly different (P＞0.05).Serum levels of IL-2 and TNF-α were markedly higher than those of ethanol group and saline groups (P＜0.05). The mean apoptotic index (AI: percentage of TUNEL signal positive cells)in Star-99 group (48.98±5.09 %) was significantly higher than that of the ethanol group (11.95±2.24 %) and the saline group (10.48±3.85 %) (P＜0.01). FCM DNA analysis showed that the appearance rate of the apoptosis peak in Srar-99group was 92.9 %, markedly higher than that of the ethanol group (14.3 %) and the saline group (0.0 %) (P＜0.01).Correlation (r=0.499, P＜0.05) was found between AI and serum level of TNF-α.CONCLUSION: Star-99 has an effect on the elevation of the serum levels of IL-2 and TNF-α. ft indi
AIM:To investigate the anti-cancer effect and the immunological mechanism of ultrasound-guided intratumoral injection of Chinese medicine'Star-99'in hepatocellular carcinoma(HCC)of nude mice. METHODS:Twenty-eight human hepatocellular carcinoma SMMC-7721 transplanted nude mice,14 of hypodermically implanted and 14 of orthotopic liver transplanted,were randomly divided into three groups of which 14 mice with Star-99,and 7 with ethanol and saline respectively.Ten clays after the transplantation the medicines were injected into the tumors of all the nude mice once every 5 days. After 4 injections the nude micewere killed.The diameters of three dimension of the tumors were measured by high frequency ultrasound before and after the treatment and the tumor growth indexes*(TGI)were calculated. Radioimmunoassay was used to detect the serum levels of interleukin-2(IL-2)and tumor necrosis factor(TNF)-alpha. The tumor tissues were sent for flow cytometry(FCM)DNA analysis.Apoptotic cells were visualized by TUNEL assay. All the experiments were carried out by double blind method. RESULTS:The TGI of Star-99 group(0.076±0.024)was markedly lower than that of the saline group(4.654±1.283) (P<0.01).It also seemed to be lower than that of the ethanol group(0.082±0.028),but not significantly different(P>0.05). Serum levels of IL-2 and TNF-α were markedly higher than those of ethanol group and saline groups(P<0.05).The mean apoptotic index(AI:percentage of TUNEL signal positive cells) in Star-99 group(48.98±5.09 %)was significantly higher than that of the ethanol group(11.95±2.24 %)and the saline group(10.48±3.85 %)(P<0.01).FCM DNA analysis showed that the appearance rate of the apoptosis peak in Srar-99 group was 92.9 %,markedly higher than that of the ethanol group(14.3 %)and the saline group(0.0 %)(P<0.01). Correlation(r=0.499,P<0.05)was found between AI and serum level of TNF-α. CONCLUSION:Star-99 has an effect on the elevation of the serum levels of IL-2 and TNF-α.It indicates that Star- 99 has the function of enhancing
