摘要
通过体外重组的方法 ,将asd基因插入重组表达质粒 ,使抗生素抗性失活 ,并与弗氏志贺氏菌FWL0 1构成宿主 载体平衡致死系统 .通过蛋白质印迹结果表明 ,在没有抗生素条件选择的情况下 ,可稳定表达肠毒素大肠杆菌定居因子抗原CS6 .重组菌通过口服和鼻饲免疫小鼠后 ,可以诱生CS6血清IgG抗体 ;同时可以检测到分泌型IgA产生 ,表明重组菌可以诱导相应的黏膜免疫反应 .
A host plasmid lethal balancing system was constructed based on asd gene in an avirulent strain of S.flexneri to express coli surface antigen 6 of enterotoxigenic Escherichia coli . The results of Western blotting demonstrated that avirulant strain of S.flexneri FWL01 expressed CS6 steadily. Immunofluorescence analysis showed that S.flexneri FWL01 carrying the plasmid pZLG6 can be excited fluorescence on its surface. Antibodies against CS6 and LPS of Shigella can be detected in sera of mice immunized with recombinant bacteria either orogastrically (o.g.) or intranasally(i.n.); simultaneously sIgA against CS6 can also be detected in the intestine. This is helpful for constructing multivalent recombinant vaccine for prevention of bacterial diarrhea.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2004年第2期154-158,共5页
Progress In Biochemistry and Biophysics
基金
国家高技术"863"计划 ( 2 0 0 1AA2 15 2 11)
全军科研基金重点课题资助项目 ( 0 1Z0 2 6)~~
