摘要
从成都井研生猪屠宰厂等地采集的样品中平板分离粗筛到 4 8株硫酸软骨素裂解酶 ABC产生菌 ,进一步复筛出一株高产、稳定的硫酸软骨素裂解酶 ABC产生菌株 GT38,初步鉴定为彭氏变形菌 (P.pen-neri)。通过单因素实验和正交分析研究了摇瓶最适产酶条件和 2 L 实验室小罐发酵工艺 ,该菌的最适发酵工艺为培养基大豆蛋白胨 2 .5 % ,蔗糖 1.5 % ,Na Cl0 .4 % ,硅油 0 .0 5 % ,p H7.5 ,接种量 5 % ,通气量 1∶ 1～1∶ 1.2 (v/ v) ,搅拌转速为 6 0 0 r/ min,30℃发酵 10 h,酶活力单位高达 32 2 U/ L。
A chondroitinase ABC producing strain was screened and isolated from slaughterhouse samples. The strain was identified as Protens penneri , called GT38. The optimum condition of the culture for the chondroitinase ABC production consisted of 2.5% soytone,2% sucrose;0.4% NaCl and 0.05% silicon oil. After fermentation in 2L-fermentor at 30℃,with an aeration rate of 1∶1～1∶1.2(v/v) 600r/min of stirring rate and fermented at 30℃ for 10 hours;the enzyme activity was about 322U/L.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
2004年第3期138-141,共4页
Chinese Journal of Antibiotics
