摘要
目的以微生物为模型 ,对柳胺酚的二相药物代谢产物进行研究。方法选用 7株丝状真菌为转化菌株 ,利用液相色谱 质谱联用技术对药物代谢产物进行检测。结果柳胺酚经过所选真菌转化后 ,形成葡萄糖结合物和五碳糖结合物 2种二相药物代谢结合产物 ,其中葡萄糖结合物为主要产物 ,产率最高可达 83 %左右 ;且无一相药物代谢产物形成。结论利用特定的微生物可建立研究柳胺酚二相代谢适宜的体外模型。
Objective To study phase Ⅱ metabolites of osalmide in the microbial model. Method Metabolites produced by 7 filamentous fungi screened were identified by liquid chromatography-tandem mass spectrometry (LC-MSn). Result Transformed by 7 fungi screened, the osalmide was converted to two phase Ⅱ metabolites:glucosides and pentosides,in which glucosides were the major product with the highest yield of 83%. In addition,no phase Ⅰ metabolites were produced. Conclusion The specific microorganism may serve as useful models for the study on phase Ⅱ metabolism of the osalmide.
出处
《沈阳药科大学学报》
CAS
CSCD
2004年第2期141-144,共4页
Journal of Shenyang Pharmaceutical University
基金
国家自然科学基金资助项目 ( 3 993 0 1 80 )
关键词
柳胺酚
微生物转化
二相药物代谢产物
小克银汉霉菌
液相色谱-串联质谱分析
osalmide
microbial transformation
phase Ⅱ metabolites
Cunninghamella species
liquid chromatography-tandem mass spectrometry
