摘要
目的 探讨免疫固定电泳技术在鉴定M蛋白上的应用。方法 采用琼脂糖凝胶免疫固定技术 (IFE)和速率散射比浊法检测了 43例病人和 2 0例正常健康人血清M蛋白。结果 其中 2 9例病人检出M蛋白 ,M蛋白分型为IgGκ型2 0例 ,IgGλ型 5例 ,IgMκ型 2例 ,IgAλ型 1例 ,单纯λ型 1例 ;14例其它疾病未检出M带。结论 采用免疫固定电泳技术 ,沉淀带易识别 ,操作简单、快速。对鉴定各类型M蛋白有重要价值。
Objective To study the application of immunofixation electrophoresis(IFE) in typing M proteins. Methods Serum M proteins were detected in 43 patients and 20 normal controls by agarose gel IFE and rate nephelometery. Results Of 43 patients, 29 were affirmed to have M proteins, including 20 cases of IgG κ, 5 IgG λ, 2 IgM κ, 1 IgA λ and 1 λ. Conclusion The technology of IFE, characterized by easy differentiation of electrophoretic bands, simple operation and rapidity, has great value in typing M proteins.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2003年第7期623-624,共2页
Journal of Third Military Medical University
关键词
免疫固定电泳
M蛋白
免疫球蛋白
immunofixation electrophoresis
M proteins
immunoglobin
