摘要
目的 研究碱性成纤维细胞生长因子 (b FGF)复合缓释降解膜对减轻或防止肌腱粘连形成的作用。 方法 用 b FGF和能促进胶原纤维合成的试剂制成 b FGF复合缓释降解膜。选取 6 6只 SD大鼠随机分成 3组 ,每组 2 2只 ,将左肢跟腱切断缝接。 A组 :切断缝接处包裹 b FGF复合缓释降解膜 ;B组 :切断缝接处只包裹单纯的降解膜 ;C组 :切断缝接处不加任何处理。术后 90天 ,对标本进行光镜、电镜、图像分析观察 ,羟脯氨酸 (HYP)含量、腱周粘连定量测定 ,及生物力学测试。 结果 A组肌腱缝合段内的成纤维细胞和胶原纤维明显较其腱周结缔组织和 B、C两组缝合段的数量多 ,差异有统计学意义 (P<0 .0 5 ) ;HYP含量和最大抗拉强度均明显高于 B、C两组 ,差异有统计学意义 (P<0 .0 1)。A组肌腱缝合段外周几乎保持原有的腱旁疏松结缔组织 ,与腱周分界清楚 ;而 B、C两组肌腱缝合段外周的腱旁组织几乎均变成较致密的结缔组织 ,并长入腱内 ,与腱周分界不清。腱周粘连定量测定结果显示 :A组腱周粘连程度远较 B、C两组轻。 结论 b FGF复合缓释降解膜 ,可使损伤肌腱内部愈合快于腱周结缔组织增生 ,有减轻或防止粘连形成的作用。
Objective To study the adhesion-preventing effect of basic fibroblast growth factor(bFGF) combined slow-releasing degradable membrane. Methods The bFGF combined slow-releasing degradable membrane was made from bFGF and the reagent which could promote fibrinogen synthesize. Sixty-six SD rats were divided into groups A,B,C randomly (22 rats each group). In group A, sutured achilles tendon were encapsulated with bFGF combined slow-releasing degradable membrane;in group B, sutured achilles tendon were encapsulated with degradable membrane without any drug; in group C, achilles tendon were only sutured. Ninety days later, light-microscope, electron-microscopoe, figure-analysing, hydroxyproline content, extent of peritendon adhesion and biomechanic test were evaluated. Results ①The amount of fibroblast and fibrinogen inside the sutured tendon in group A was larger than that in its peripheral connective tissue and in groups B and C (P<0.05). The content of hydroxyproline and the ultimate tensile strength in group A was higher than those in groups B and C(P<0.01).② The peripheral tissue in group A almost remains the formal loose connective tissue, but it became dense connective tissue in groups B and C and grew into the tendon. Moreover, the extent of adhesion in group A was lesser than that in groups B, C according to the mensuration of peritendon adhesion. Conclusion The bFGF combined slow-releasing degradable membrane can make the intrinsic healing of tendon faster than peripheral connective tissue proliferation, so it can prevent the formation of adhesion.
出处
《中国修复重建外科杂志》
CAS
CSCD
2004年第2期148-151,共4页
Chinese Journal of Reparative and Reconstructive Surgery
