摘要
目的 :建立一种简便、实用的亲缘关系相近的茵陈类药材DNA分子鉴定方法 ;找出中韩两国茵陈药材在遗传学上的差异。方法 :采用聚合酶链反应产物直接测序法对 3种茵陈 (茵陈蒿、韩茵陈和白莲蒿 )进行鉴别。从 3种茵陈的组织材料中提取DNA ,扩增rDNA的内转录间隔区 (internaltranscribedspacers ,ITS)和 5 .8s的序列。对扩增产物进行了DNA序列分析。结果 :韩茵陈、白莲蒿在PCR产物凝胶电泳图谱上有明显差异 ,且DNA序列分析结果差异为 3.96 % ,韩茵陈和茵陈蒿在遗传学上存在差异。结论 :用rDNA的ITS序列分析法鉴别茵陈类药材 ,方法简便。
Objective:To make an useful identification method for the molecule of DNA on 3 herbs of Artemisia genus and compare the differences of the genes of Korean and Chinese species of Artemisia. Methods:Sequence of 3 herbs (Artemisia sacrorum Ledeb., Artemisia iwayomogi Kitam. and Artemisia capillaris Thunb.) was determined by PCR sequence system. DNA was extracted from rDNA/ITS (internal transcribed spacers) and 5.8s. The analysis was based on the amplification through DNA sequence system. Results:There were profound differences between the Korean Artemisia and Artemisia sacrorum L.. These 2 herbs had a difference in the PCR amplifications of the agarose gel electrophoresis. There was a slight difference in the analysis of the DNA sequence system, and the substitution percentage for ITS gene fragments sequence was 3.96%. Conclusion:Analytic identification method on sequence system of ITS in rDNA is effective for these 3 herbs.
出处
《中西医结合学报》
CAS
2004年第1期58-61,共4页
Journal of Chinese Integrative Medicine
关键词
韩茵陈
药材基源
rDNA内转录
序列分析
聚合酶链反应
中药
Artemisia iwayomogi Kitam.
Artemisia sacrorum Ledeb.
Artemisia capillalis Thunb.
molecular identification
polymerase chain reaction
