摘要
目的 :建立一种简便实用的白花蛇舌草药材的DNA分子鉴定方法。方法 :收集目前市场上出现的白花蛇舌草及伪品共 9种 ,分别提取总DNA ,扩增rDNAITS 2区序列 ,对比所有样品的该段核苷酸序列 ,并设计能专一性鉴别白花蛇舌草的位点特异性引物。结果 :白花蛇舌草及其混淆品在ITS 2区的序列有显著差异。用位点特异性引物对所有样品DNA进行PCR扩增 ,只有白花蛇舌草有明显的约 392bp扩增产物 ,而其它 8种植物在同等条件下无阳性产物。结论 :所设计的鉴别引物对白花蛇舌草有高度的特异性。
Objective: To develop a convenient and effective method for the identification of Hedyotis diffusa. Methods: DNA templates were extracted from H. diffusa and its adulterants samples on commercial markets. And DNA fragments of rDNA ITS 2 regions were amplified and sequenced subsequently. ITS 2 sequences of all samples were aligned. The allele specific primer was designed for distinguishing H. diffusa. Results: The nucleotide difference between H. diffusa and 8 other species is obvious in the ITS 2 region. In addition, the allele specific primers were employed to amplify the DNA from H. diffusa and 8 other species. The result indicated that a 392 bp DNA fragment was amplified from H. diffusa, whereeas no any fragment was amplified from 8 other species under the same reaction condition. Conclusion: The primers designed in the present study were highly specific for H. diffusa. They could be used as key components in the H. diffusa identification kit.
出处
《中药材》
CAS
CSCD
北大核心
2004年第7期484-487,共4页
Journal of Chinese Medicinal Materials
基金
江苏省科技厅社会发展基金 (BS2 0 0 10 16)资助
