摘要
使用高度特异的BrdU抗血清,通过酶标第二抗体和DAB-H_2O_2或AEC-H_2O_2显色法,成功地显示了CHO和人染色体的BrdU标记区域。使用同样的程序和方法亦成功地显示了硝酸纤维膜上pg水平的BrdU-DNA印迹点。
Utilizing highly specific BrdU antiserum, via the second antibody conjugated with enzyme and DAB-H2O3 or AEC-H2O3 colorific methods, the BrdU marked regions of CHO and human chromosomes were detected clearly. The BrdU-DNA prints (pg) on nitrocellulose membranes were also showed with similar methods.
