摘要
为研究“补肾活血”对老年鼠脑乙酰胆碱 (Ach)代谢的影响 ,将老年大鼠分别用补肾益精、行气活血、补肾活血方剂灌胃 30d ,采用高效液相色谱技术检测大鼠脑内Ach摩尔质量浓度、分光光度法检测胆碱乙酰转移酶 (ChAT)和胆碱酯酶 (AChE)的活性 .研究发现老年大鼠脑内Ach摩尔质量浓度、ChAT活性显著低于青年鼠 ,AChE活性显著高于青年鼠 ;补肾益精、行气活血、补肾活血均可改善老年鼠脑内Ach代谢 ,补肾活血法明显优于补肾益精法。
To investigate the effect of different therapeutic st ra tegies (bushen-yijing, Xingqi-huoxue and bushen-huoxue) on the metabo lism of acetylcholine (Ach) in brains of old rats, different prescriptions were offered to old rats for 30 days according to different therapeutic strategies me ntioned above. The level of Ach was detected by high performance liquid chromato graphy and the activities of choline acetyltransferase (ChAT) and acetylcholines terase (AChE) in rat brains were assayed by spectrophotometry. These Ach level a nd the activity of ChAT were decreased, and the activity of AChE was increased i n brains of old rats. These three therapeutic strategies were proved to have ame liorated the metabolism of Ach in the brains of old rats and bushen-huoxue was the best one.
出处
《华中科技大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2004年第2期92-94,共3页
Journal of Huazhong University of Science and Technology(Natural Science Edition)
基金
国家自然科学基金资助项目 ( 30 70 56 0
3992 50 12
30 10 0 0 57
30 170 2 2 1)
关键词
乙酰胆碱
胆碱乙酰转移酶
胆碱酯酶
中医药治疗
实验研究
acetylcholine
choline acetyltransferase
a cetylcholinesterase
Chinese medicine therapy
experimental investigation
