摘要
目的 :克隆和序列分析中国猪种DQA基因cDNA ,为猪异种器官移植的免疫识别机理的研究提供基础。方法 :采用RT PCR方法扩增广西巴马猪 (GXP)、贵州香猪 (GZP)及云南小耳猪 (YNP)DQA基因cDNA ,克隆入测序载体 ,然后进行测序及其序列分析。结果 :获得具有阅读框架的 3个结构和已有序列不同的DQA新等位基因 (GXPDQA、GZPDQA和YNPDQA) ,基因序列号分别为AY10 2 4 73,AY10 2 4 74和AY10 2 4 75 ,其长度分别为 76 5、76 8和 76 8个核苷酸 ,除末端终止密码外 ,分别编码2 5 4、2 5 5和 2 5 5个氨基酸残基。结论 :发现了 3个猪DQA新等位基因 ,同时发现中国猪种DQA基因及其推导的氨基酸与人相应基因的同源性明显高于小鼠。
Objective:To lay background for studying rejection mechanisms in xenotransplantation, class II DQA genes of swine leukocyte antigens (SLA) of three Chinese pig strains GXP, GZP and YNP were cloned and sequenced.Methods:RT-PCR was performed to proliferate SLA-DQA genes (GXPDQA, GZPDQA and YNPDQA),which were cloned into pGEM-T Easy vector for sequencing and analysis.Results:All three allelic sequences examined, are not identical to those reported, which allows the sequences receiving their accession numbers from GenBank as AY102473, AY102474 and AY102475, which encompass an open reading frame of 765, 768 and 768 nucleotides respectively.Conclusion:Three new alleles of SLA-DQA genes were obtained. Analysis of the amino acid sequences of swine, human and mouse DQA (or equivalent) also indicated that GXPDQA, GZPDQA and YNPDQA were more similar to human DQA than mouse H-2-Aα, which might provide an understanding of how the immune system evolved.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2004年第2期111-116,共6页
Chinese Journal of Immunology
基金
国家自然科学基金资助重大项目 (批准号 :3 9993 43 0 2 )
关键词
猪白细胞抗原DQA
主要组织相容性复合体
异种移植
Swine leukocyte antigen (SLA) DQA gene
Major hisocompatibility complex
Xenotransplantation
