摘要
目的 比较人眼滴用氧氟沙星、环丙沙星及妥布霉素的前房穿透性差异。方法 2 0 0 3年 4～ 8月在我院门诊预行白内障超声乳化术的老年白内障患者 12 5例 (12 5只单侧眼 ) ,使用随机数字表分为 3个大组 :氧氟沙星组 (42只眼 ) ,环丙沙星组 (41只眼 ) ,妥布霉素组 (42只眼 ) ,每个大组再使用随机排列表分为 5个小组 ,每个小组 8或 9只眼。按不同大组 ,术前局部给予 0 3%氧氟沙星、0 3%环丙沙星或 0 3%妥布霉素 ,滴眼 6次 ,每次间隔 15min。手术时按不同小组 ,于最后 1次给药后 7 5、15 0、30 0、6 0 0、12 0 0min时抽取前房水约 10 0 μl。使用高效液相色谱仪分析前房水中药物浓度。结果 各时间点前房内氧氟沙星浓度均明显高于环丙沙星 ,氧氟沙星生物利用度是环丙沙星的 6倍。高效液相色谱仪未能检测到妥布霉素 ,故其浓度低于最低检测限 0 0 5 μg/ml。 结论 人眼滴用氧氟沙星、环丙沙星和妥布霉素的结果表明氧氟沙星前房穿透性最好 ,提示可作为预防和治疗眼内炎的首选局部用药。
Objective To compare the aqueous humor penetration of topically applied ofloxacin, ciprofloxacin and tobramycin in human eyes. Methods Senile cataract patients (125 eyes in 125 cases) receiving phacoemulsification were randomized into three groups: ofloxacin, 42 eyes; ciprofloxacin, 41 eyes and tobramycin, 41 eyes. Each group was randomized into five sub-groups, with 8 or 9 eyes in each sub-group. Each group received either 0.3% ofloxacin, ciprofloxacin or tobramycin topical drops preoperatively. Eyedrops were instilled one drop every 15 minutes for six times. Aqueous samples were obtained from different sub-groups during surgery, 7.5, 15.0, 30.0, 60.0 and 120.0 min after the last drop, respectively. Concentration of the antimicrobial agents was determined by the HPLC (high pressure liquid chromatography). Results Aqueous humor concentration of ofloxacin was significantly higher than that of ciprofloxacin at the same period after the last drop. The bioavailability of ofloxacin was 6 times that of ciprofloxacin. Tobramycin could not be detected by HPLC, so its concentration was lower than the minimum detectable level by HPLC (0.05 μg/ml). Conclusion Ofloxacin has the best aqueous penetration in human eyes, as compared with ciprofloxacin and tobramycin, which suggests ofloxacin could be the preferred topical medicine for the prevention and treatment of endophthalmitis.
出处
《中华眼科杂志》
CAS
CSCD
北大核心
2003年第12期736-739,共4页
Chinese Journal of Ophthalmology
