摘要
目的 :建立采用高效液相色谱法 (HPLC)测定联磺甲氧苄啶片中磺胺甲卟恶唑 ,磺胺嘧啶和甲氧苄啶的含量。方法 :色谱柱为Diamonsil (TM)C1 8(5μm ,2 0 0mm×4 .6mm) ,流动相为乙腈∶水∶磷酸 (50∶50∶0 .1 ) ,每 1 0 0 0mL流动相中加十二烷基硫酸钠 3 .5g,流速 1mL·min- 1 ,检测波长为 2 57nm。结果 :磺胺甲卟恶 唑、磺胺嘧啶和甲氧苄啶 3种成分的回收率分别为99.78% ,99.82 % ,99.90 %。结论 :方法简便 ,结果准确可靠。
OBJECTIVE To establish an external standard HPLC method for the determination of sulfamethoxazole, sulfadiazine and trimethoprim in sulfamethoxazole,sulfadiazine and trimethoprim tablets.METHODS The chromatographic coditions were as follows: Diamonsil (TM) C 18 (5 μm, 200 mm × 4.6 mm ), acetonitrile-water-phosphoric acid(50∶50∶ 0.1 ) as mobile phase.The mobile plase contained 3.5 g of sodium laury sulfate ,flow rate was 1 mL ·min -1 ,detection wavelength was 257 nm .RESULTS The mean recoveries of sulfamethoxazole sulfadiazine and metodopramide were 99.78% , 99.82% , 99.90% ,respectively.CONCLUSIONS This method is simple, accurate.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2004年第2期81-83,共3页
Chinese Journal of Hospital Pharmacy
