摘要
目的:研究HPLC法同时测定联磺甲氧苄啶片中磺胺甲噁唑、磺胺嘧啶和甲氧苄啶的含量。方法:采用高效液相色谱法,色谱柱为C18柱(4.6 mm×250 mm,5μm),以0.025 mol/L磷酸溶液-乙腈(80∶20)为流动相,检测波长为220 nm。结果:磺胺甲噁唑、磺胺嘧啶和甲氧苄啶的线性范围分别为40￣240μg/ml(r=0.9999)、40￣240μg/ml(r=0.9999)和16～96μg/ml(r=0.9995),回收率分别为100.4%(RSD=0.30%)、100.4%(RSD=0.21%)、101.7%(RSD=1.29%)。结论:该法快速、简便、重现性好。
Objective： To establish a method to determine the contents of three compounds in Strlfamethoxazole, Sulfadiazine and Trimethoprim Tablets. Methods： The contents were determined by HPLC with C18 column （4.6 mm×250 mm,5 μm）. The mobile phase was 0.025 mol/L phosphate solution-acetonitrile （80：20） and the detection wavelength was at 220 nm, Results： The Sulfamethoxazole, Sulfadiazine and Trimethoprim was linear within the ranges of 40 - 240 μg/ml （r = 0.9999）, 40 - 240 μg/ml （r = 0.9999） and 16 - 96 μg/ml （r = 0.9995） respectively. The average recovery was 100.4% （RSD = 0.30%）, 100.4% （RSD = 0.21%） and 101.7% （RSD = 1.29%）, respectively. Conclusion： This method is fast, simple and reproducible.
出处
《儿科药学杂志》
CAS
2005年第5期43-44,共2页
Journal of Pediatric Pharmacy
关键词
高效液相色谱法
联磺甲氧苄啶片
磺胺甲噁唑
磺胺嘧啶
甲氧苄啶
HPLC
Sulfamethoxazole, Sulfadiazine and Trimethoprim tablets
Sulfamethoxazole
Sulfadiazine
Trimethoprim
