摘要
以‘津优35号’黄瓜(Cucumis sativus)为试材,采用营养液水培,用聚乙二醇(PEG) 6000模拟干旱条件,研究了CO2加富对干旱胁迫下黄瓜幼苗根系膜脂过氧化、活性氧(ROS)积累及其清除能力的影响。结果表明:干旱胁迫降低根系生物量及根系活力,提高根冠比,增加丙二醛(MDA)含量,促进ROS的累积,提高脯氨酸(Pro)、还原型抗坏血酸(AsA)、还原型谷胱甘肽(GSH)含量及抗氧化酶活性;而CO2加富处理显著增加干旱胁迫下根系生物量,提高根冠比,促进根系活力增加,降低超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性,提高抗坏血酸过氧化物酶(APX)和谷胱甘肽还原酶(GR)活性,提高AsA、GSH及Pro含量,使得MDA含量以及ROS产生速率显著降低。综上所述, CO2加富可提高干旱胁迫下黄瓜根系生物量及根系活力,进而增强根系的吸收能力,并能够在一定程度上激活根系抗氧化防御系统,抑制ROS的积累,防止膜脂过氧化,从而缓解干旱胁迫对黄瓜幼苗根系造成的不利影响。
We used hydroponic‘Jinyou 35’cucumber(Cucumis sativus)seedlings as materials to explore the effect of elevated CO2 concentration on membrane lipid peroxidation,accumulation and removal rate of reactive oxygen species(ROS)under drought stress.The results show that drought stress could significantly reduce the biomass and activity of root,increase root/shoot ratio,accumulation of malondialdehyde(MDA)and ROS,contents of proline(Pro),ascorbic acid(AsA)and glutathione(GSH),and antioxidant enzyme activities.However,the elevated CO2 concentration significantly enhanced the biomass and activity of root,improved the root/shoot ratio,reduced activities of superoxide dismutase,peroxidase and catalase,increased activities of aseorbate peroxidase and glutathione reductase as well as contents of AsA,GSH and Pro,and significantly reduced MDA content and ROS production rate under drought stress.In summary,elevated CO2 concentration could increase biomass and activity of cucumber seedling roots,then enhance root absorption capacity,activate root antioxidant system to some extent,inhibit ROS accumulation and prevent membrane lipid peroxidation,and consequently alleviate the adverse effect of drought stress on cucumber seedling root.
作者
李仪曼
贺新蕊
李清明
刘彬彬
厉书豪
艾希珍
魏珉
张大龙
LI Yi-Man;HE Xin-Rui;LI Qing-Ming;LIU Bin-Bin;LI Shu-Hao;AI Xi-Zhen;WEI Min;ZHANG Da-Long(College of Horticulture Science and Engineering,Shandong Agricultural University,Taian,Shandong 271018,China;State Key Laboratory of Crop Biology,Taian,Shandong 271018,China;Scientific Observing and Experimental Station of Environment Controlled Agricultural Engineering in Huang-Huai-Hai Region,Ministry of Agriculture,Taian,Shandong 271018,China)
出处
《植物生理学报》
CAS
CSCD
北大核心
2019年第7期1011-1019,共9页
Plant Physiology Journal
基金
国家自然科学基金(31471918和31872154)
国家科技支撑计划项目(2014BAD05B03)
山东省重点研发计划项目(2017CXGC0201)
山东省农业重大应用技术创新项目(鲁财农指[2016]36号)
山东农业大学科技创新团队设施园艺优势团队项目(SYL2017YSTD07)~~
关键词
CO2加富
黄瓜根系
干旱胁迫
抗氧化系统
CO2 enrichment
cucumber root system
drought stress
antioxidant system
