摘要
以枯草芽胞杆菌母药为研究对象,通过对载体、分散剂、表面活性剂、保护剂的筛选,确定了枯草芽胞杆菌微粉剂的最佳配方。其配方为:枯草芽胞杆菌母药50%、载体白炭黑20%、分散剂萘磺酸钠盐甲醛缩合物(NNO) 2%、表面活性剂烷基萘磺酸盐(EFW) 3%、保护剂羧甲基纤维素(CMC) 1.5%、载体硅藻土补足至100%。测定结果表明:枯草芽胞杆菌微粉剂含菌量为2.11×1010CFU·g-1,分散指数89.56,浮游指数87.57,坡度角74°,pH 6.1,杂菌率2.1%,含水率0.67%,粒径8.15μm,热贮分解率为4.67%,产品各项指标均达到标准。盆栽条件下,枯草芽胞杆菌微粉剂用量1 000 g·(hm2)-1时喷粉对黄瓜白粉病的保护效果优于治疗效果,防效分别为89.08%和84.95%;田间试验,枯草芽胞杆菌微粉剂在用量为1 000 g·(hm2)-1时喷粉对黄瓜白粉病的保护效果为80.66%。
By screening the carriers,dispersing agents,surface active agent and protection agents for Bacillus subtilis micropowder preparation,the optimal formulation were confirmed as follows:50.0%the content of Bacillus subtilis mother powder,20.0%filler carbon-white and diatomite,2.0%dispersing agent NNO,3.0%surface active agent EFW,1.5%UV protection agents CMC,Carrier diatomite was supplemented to 100%.The quality detection showed that the spores content of B.subtilis micropowder could come up to 2.11×1010 CFU·g-1,the p H value was 6.1,the rate of heterozygous bacteria was 2.1%,the water content was 0.67%,Particle size 8.15μm,the spore dispersion index was 89.56,the floatation index is 87.57,the slope angle is 74°,and thermal decomposition rate of storage was 4.67%,which came up to the formulation standard.Pot experiment demonstrated that the protective effects of B.subtilis micropowder on cucumber powdery mildew was better than the therapeutic effect when the dosage of Bacillus subtilis powder was 1 000 g·(hm2)-1,and the control effects were89.08%and 84.95%,respectively.In the field experiment,the protective effect of B.subtilis micropowder on cucumber powdery mildew at 1 000 g·(hm2)-1 was 80.66%.
作者
揣红运
谢学文
石延霞
柴阿丽
李宝聚
CHUAI Hong-yun;XIE Xue-wen;SHI Yan-xia;CHAI A-li;LI Bao-ju(Plant Protection College,Shenyang Agricultural University,Shenyang 110866,China;Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China)
出处
《植物病理学报》
CAS
CSCD
北大核心
2019年第5期660-669,共10页
Acta Phytopathologica Sinica
基金
国家重点研发计划(2018YFD0201300)
农业部园艺作物生物学与种质创制重点实验室项目
关键词
枯草芽胞杆菌
微粉剂
黄瓜白粉病
防治效果
Bacillus subtilis
micropowder
cucumber powdery mildew
control effect
