摘要
植原体是一类重要的植物病原菌,属柔膜菌纲、植原体属,没有细胞壁,专性寄生在植物的韧皮部筛管细胞内[1]。枣疯病(Jujube witches’ broom,JWB)是枣树的重要病害之一,对枣树种植产业具有毁灭性的危害。枣疯病在我国分布广泛,从四川、重庆到河北、山东、山西和北京都有分布,而在华南地区暂无报道。
Several jujube plants with witches’broom,little leaf,and big bud symptoms,which were likely infected by jujube witches’broom(JWB)phytoplasma,were collected in Guangzhou,Guangdong Province.To identify the pathogen,PCR was performed using phytoplasma 16S rDNA universal primer pairs R16 mF2/R1 and P1/P7 and SecA gene primer pair SecAfor1/rev3 with total DNA of the symptomatic plants as templates.Specific fragments,1.4 kb,1.8 kb,and 0.8 kb in length,were amplified from one of three symptomatic samples.Phylogenetic analysis based on 16S rDNA verified that the pathogen harming jujube plants in Guangzhou was jujube witches’broom phytoplasma which belonged to 16S rV-B subgroup.Comparison results also showed that the 16S rDNA sequence of Guangzhou JWB phytoplasma shared the highest nucleotide identity(100%)with the reported jujube witches’broom phytoplasma Japanese strain(AB442218)and JWB strain(AY197661)and shared the nucleotide identity ranging from 99.74%to 99.80%with the other JWB phytoplasma strains.In addition,phylogenetic analysis based on SecA also showed that Guangzhou jujube witches’broom phytoplasma belonged to 16S rV-B subgroup and shared 99.28%-99.76%similarity with other phytoplasma strains.All these results suggested that jujube witches’broom phytoplasma has infected jujube plants in Guangdong Province.
作者
李正刚
佘小漫
汤亚飞
于琳
蓝国兵
邓铭光
何自福
LI Zheng-gang;SHE Xiao-man;TANG Ya-fei;YU Lin;LAN Guo-bing;DENG Ming-guang;HE Zi-fu(Plant Protection Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China;Guangdong Provincial Key Laboratory of High Technology for Plant Protection,Guangzhou 510640,China)
出处
《植物病理学报》
CAS
CSCD
北大核心
2019年第2期281-282,共2页
Acta Phytopathologica Sinica
基金
国家重点研发计划(2017YFC1200600)
广东省现代农业科技创新联盟建设项目(2017LM2150)
