摘要
为进行一例鸡源致病型大肠杆菌的分离鉴定,无菌采集疑似大肠杆菌患病蛋鸡肝脏、脾脏等组织,进行病原菌的分离培养。利用培养特性检查、菌落形态观察、革兰氏染色鉴定、生化特性考察、PCR鉴定、测序分析以及动物致病性试验对分离菌株进行鉴定,并同时进行药敏试验。结果显示,分离菌株为一株致病型大肠杆菌,对青霉素、氟苯尼考、阿莫西林等多种抗生素产生耐药性,对丁胺卡那敏感。建议使用丁胺卡那霉素对大肠杆菌进行防治。
In order to isolate and identificate a chicken-derived pathogenic Escherichia coli,the liver and spleen of the chicken suspected E.coli were collected aseptically,and the pathogens were isolated and cultured.The culture characteristics examination,colony morphology observation,gram stain identification,biochemical characteristics investigation,PCR identification,sequence analysis and animal attack test were used to identificate.At the same time,the susceptibility test was carried out on the isolated strain.The results showed that the isolated strain was Escherichia coli,which was resistant to various antibiotics such as penicillin,florfenicol and amoxicillin,and sensitive to amikacin.Amikacin was recommended to prevent the E.coli.
作者
朱庆贺
王爽
李文超
陈曦
杨旭东
王观悦
李丹
史同瑞
ZHU Qing-he;WANG Shuang;LI Wen-chao;CHENG Xi;YANG Xu-dong;WANG Guan-yue;LI Dan;SHI Tong-rui(Heilongjiang Institute of Veterinary Medicine Science,Heilongjiang,Qiqihaer161005,China;Animal Husbandry Bureau of Fangzheng County,Harbin150800,China)
出处
《中国兽药杂志》
2019年第9期7-11,共5页
Chinese Journal of Veterinary Drug
基金
黑龙江省应用技术研究与开发计划(sczzn2018-2)
关键词
蛋鸡
大肠杆菌
分离鉴定
药敏试验
hens
E.coli
separation and identification
drug sensitive test
