摘要
按照MALDI-TOF MS要求进行56株鸡杆菌分离株的样品制备,点靶并获取样品质谱图,运用Biotyper分析软件对所获取的质谱图鉴定分析;同时对这些菌株进行荧光定量PCR检测、gyrB基因的PCR扩增和序列分析,进行辅助分析鉴定。结果显示,56株鸡杆菌分离株经MALDI-TOF MS和gyrB基因序列分析均鉴定为鸭源鸡杆菌,阳性率为100.0%;荧光定量PCR鉴定出55株鸭源鸡杆菌,阳性率为98.2%(55/56)。结果表明,MALDI-TOF MS可用于鸭源鸡杆菌分离株的快速鉴定,操作简便,准确率高。
Matrix-assisted laser desorption/ionization time of flight mass spectrometry(MALDITOF MS)was applied for rapid identification of 56 Gallibacterium strains.The bacteria sample were prepared by method of MALDI-TOF MS,then added to the target polished steel plate and the mass spectra were acquired.The spectra were analyzed by the Biotyper analysis software.At the same time,these strains were identified by real-time fluorescence quantitative PCR and gyrB gene sequence analysis,for auxiliary analysis and identification.All 56strains were identified as Gallibacterium anatis rapidly by MALDI-TOF MS,showing 100.0%positive rate,which is consistent with the identification of gyrB gene sequence analysis.55strains of Gallibacterium anatis were identified by real time fluorescence quantitative PCR with a 98.2%(55/56)detectionA3rate.MALDITOF MS can be used to identify Gallibacterium anatis,as a fast,simple,accurate diagnostic tools in veterinary clinic.
作者
王海花
皇甫和平
李文刚
苗丽
董青
孙彦婷
郭宏伟
王宏魁
杜振隆
贾含笑
WANG Hai-hua;HUANG FU He-ping;LI Wen-gang;MIAO Li;DONG Qing;SUN Yan-ting;GUO Hong-wei;WANG Hong-kui;DU Zhen-long;JIA Han-xiao(Henan University of Animal Husbandry and Economy,Zhengzhou 450046,China;Henan Entry-Exit Inspection and Quarantine Bureau,Zhengzhou 450003,China)
出处
《中国兽医学报》
CAS
CSCD
北大核心
2019年第2期234-238,共5页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目(U1404328)
河南牧业经济学院科技创新团队资助项目(HUAHE2015014)
河南牧业经济学院预防兽医学重点学科资助项目(MXK2016102)
国家质量监督检验检疫总局科技计划资助项目(2015IK113)
