摘要
利用可培养组技术,通过设计和优化筛选培养基,结合基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorption/ionization time of flight mass spectrometry,MALDI-TOF MS)和16S rDNA基因鉴定技术,大规模筛选鉴定浓香型白酒窖池中可培养细菌,重点突破厌氧菌的分离培养。研究结果表明,采用5种特定的厌氧培养基和2种好氧培养基,从浓香型白酒窖池中共分离得到了来自21个属44个种的124株细菌,包括9株潜在细菌新种。其中,厌氧条件分离得到100株,好氧条件分离得到24株;窖泥样品分离得到60株,酒醅样品分离得到64株。芽胞杆菌属(Bacillus sp.)和梭菌属(Clostridium sp.)是窖池样品中分离的优势菌种。根据不同分离筛选条件和分离生境的可培养细菌种类和丰度,确定了6株浓香型白酒窖池特征菌,并解析了特征菌的生物学特性。该研究为浓香型白酒窖池中潜在新种的挖掘,特征性菌种资源的定向筛选、互作机制解析、功能性研究开发及产业化应用奠定了基础。
Culturomics technology was used to screen and identify culturable bacteria in strong-flavor Baijiu cellars on a large scale and focus on breakthrough in the isolation and screening of anaerobic bacteria,by designing and optimizing screening medium,combined with MALDI-TOF MS and 16S rRNA gene identification technology.The results showed that a total of 124 bacteria strains from 44 species and 21 genera,including 9 potential novel species,were isolated from strong-flavor Baijiu cellars using 5 specific anaerobic medium and 2 aerobic medium.Among them,100 strains were isolated under anaerobic conditions and 24 strains were isolated under aerobic conditions;60 strains were isolated from pit mud samples and 64 strains were isolated from fermented grains samples.Bacillus sp.and Clostridium sp.were the dominant strains isolated from cellar samples.According to the species and abundance of culturable bacteria from different screening conditions and isolation habitat,six characteristic strains from strong-flavor Baijiu cellars were identified,and their biological characteristics were analyzed.This study laid a foundation for the excavation of potential novel species in strong-flavor Baijiu cellars,the directional screening of characteristic strain resources,the analysis of interaction mechanism,functional research and development,and industrial application.
作者
刘瑞娜
奚文韬
赵东
张天圆
郑佳
张京涛
姚粟
翟磊
LIU Ruina;XI Wentao;ZHAO Dong;ZHANG Tianyuan;ZHENG Jia;ZHANG Jingtao;YAO Su;ZHAI Lei(China National Research Institute of Food and Fermentation Industries Co.Ltd.,China Center of Industrial Culture Collection,Beijing 100015,China;Wuliangye Yibin Co.Ltd.,Yibin 644000,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2023年第17期82-93,共12页
Food and Fermentation Industries
基金
中国轻工业浓香型白酒固态发酵重点实验室开放基金项目(2021JJ008)。
关键词
浓香型白酒
窖泥
酒醅
可培养细菌
strong-flavor Baijiu
pit muds
fermented grains
culturable bacteria
