摘要
目的:采用网络药理学的方法,对五苓散(WLS)治疗类风湿关节炎(RA)的作用机制进行研究探讨。方法:通过在中药系统药理学数据库(TCMSP)平台对五苓散进行活性成分筛选、在Drag Bank数据库进行靶点预测,应用Cytoscape3. 2. 1软件构建成分-靶点网络;在TTD,Drug Bank及Dis Genet数据库中进行检索,获取疾病靶点;构建韦恩图,得到五苓散治疗RA的作用靶点;并进行基因本体(GO)功能注释和东京基因组百科全书(KEGG)通路富集分析;构建五苓散中药-成分-靶点-信号通路网络,使用Network Analyzer进行网络特征分析。结果:研究显示共挖掘到五苓散中52种成分及297个潜在靶点,RA疾病1 845个相关靶点,得到五苓散-疾病共同靶点49个,共同作用靶点主要富集于322个生物过程和31条信号通路上。结论:五苓散可能是通过调节前列素内环氧化物合成酶2(PTGS2),转化生长因子-β1(TGF-β1),半胱氨酸天冬氨酸蛋白水解酶-3(Caspase-3),转录因子p65(RELA),孕激素受体(PGR)等靶点,调控癌症相关通路,肿瘤坏死因子(TNF)信号通路,白细胞介素17(IL-17)信号通路,核转录因子-κB(NF-κB)信号通路,Th17细胞分化等通路,从而抑制炎症反应、调节免疫功能及调控细胞凋亡来治疗类风湿关节炎。
Objective:To study the mechanism of Wulingsan(WLS)in the treatment of rheumatoid arthritis(RA)by network pharmacology.Method:The active components of WLS were screened on traditional Chinese medicine systems pharmacology(TCMSP)platform,and the targets were predicted in DragBank database.The'component-target'network was constructed by Cytoscape 3.2.1 software.Disease targets were searched in TTD,DrugBank and Dis Genet databases.The Venn diagram was built to extract the target of WLS in the treatment of RA,and the gene oesthetics(GO)function annotation and Kyoto Encyclopedin of Genes and Genomes(KEGG)signal pathway enrichment analysis were performed by cluego plugin.The TCM-component-target-pathway network of WLS was constructed,and the network feature analysis was made by Network Analyzer.Result:Totally52 components and 297 potential targets in WLS and 1 845 targets relating to RA were excavated,and 49 common targets of WLS-RA were obtained.The common targets were mainly enriched in 322 biological processes and 31 signaling pathways.Conclusion:WLS may regulate targets,such as prostaglandin epoxide synthase 2(PTGS2),transforming growth factor-β1(TGF-β1),cysteine aspartate protein-3(Caspase-3),transcription factor p65(RELA),progesterone receptor(PGR),and adjust cancer-related pathways,tumor necrosis factor(TNF)signaling pathways,interleukin-17(IL-17)signaling pathways,nuclear factor-κB(NF-κB)signaling pathways,Th17 cell differentiation,so as to inhibit the inflammatory response,regulate immune function and adjust apoptosis to treat rheumatoid arthritis.
作者
钱凯
杜彦仪
韩隆胤
魏赈权
黄文广
曾丽盈
许舒迪
刘敏莹
林昌松
QIAN Kai;DU Yan-yi;HAN Long-yin;WEI Zhen-quan;HUANG Wen-guang;ZENG Li-ying;XU Shu-di;LIU Min-ying;LIN Chang-song(Guangzhou University of Chinese Medicine,Guangzhou510405,China;The First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou510405,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2019年第19期138-146,共9页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81573930,81774262)
关键词
五苓散
网络药理学
类风湿关节炎
靶点
信号通路
Wulingsan
network pharmacology
rheumatoid arthritis
target
signaling pathway
