摘要
为探究青岛近岸金乌贼(Sepia esculenta)繁殖群体结构及其结群历时长、产卵期内亲体规格递减的结群现象,运用形态学度量方法结合微卫星分子标记技术,对青岛薛家岛近岸前、中、后3个不同洄游时期金乌贼繁殖群体的形态特征和遗传分化水平进行统计分析,探究其亲缘关系并量化差异水平。主要结果如下:形态学主成分分析(PCA)结果显示,前3个主成分的累积贡献率为60.067%,低于85%;判别分析(D_A)结果显示, 3个时期繁殖群体的判别准确率介于66.7%～82.1%,两者的散点图结果一致,均显示3个群体未明显占据不相重叠的区域。微卫星标记结果表明, 3个时期繁殖群体的遗传距离D_A介于0.12～0.16,群体遗传分化指数为0.0014～0.0064,利用Structure判断最佳理论组群为1,表明其亲缘关系较近。综合分析认为,虽青岛近海不同洄游时期金乌贼繁殖群体的规格差异较大,但形态学参数差异较小,遗传距离较近且遗传分化水平较低(F_(st)<0.05),未呈现明显的群体分化。
Population differentiation and structure are important topics of evolutionary biology, and morphology and genetics are the main methods of researching these topics. Sepia esculenta migrates annually from the overwintering field to the spawning ground, and for a long time, it has been thought that the breeding period of its inshore populations was as long as two months. In addition, during the grouping period, the specification of S. esculenta arriving in different stages showed a decreasing trend with arrival time. Thus, the objectives of this study were to characterize the population structure and clustering phenomenon in S. esculenta over time, and discover why the parental specification diminished during spawning of the mature animals in Qingdao. The morphological characteristics and genetic differentiation during three different migratory periods(early, middle, and late) of S.esculenta inhabiting the coastal waters of Xuejia Island were analyzed using morphology and DNA markers. The results showed that the cumulative contribution rate of the first three principal components was 60.067% in the principal component analysis(PCA), which was lower than 85%. The discrimination accuracy ranged from 66.7%to 82.1% in the discriminant analysis(D_A). The scatter plots of PCA and D_A showed a similar result, which is the species was not clearly dispersed among these three migratory periods. Analysis based on microsatellite markers indicated that genetic distances(D_A) ranged from 0.12 to 0.16 and the genetic differentiation index(Fst) ranged from 0.0014 to 0.0064 in S. esculenta from the three different migratory periods. The optimal number of theoretical groups was 1 according to the genetic structural analysis. These findings suggested that there was no obvious population differentiation among early, middle, and late migratory periods of S. esculenta in Qingdao, owing to the low values of morphological differences, genetic distances, and genetic differentiation, although the population showed a large difference in mant
作者
王林龙
张秀梅
王展
宋娜
高天翔
WANG Linlong;ZHANG Xiumei;WANG Zhan;SONG Na;GAO Tianxiang(Key Laboratory of Mariculture,Ministry of Education,Ocean University of China,Qingdao 266003,China;Qingdao National Laboratory for Marine Science and Technology,Function Laboratory for Marine Fisheries Science and Food Production Processes,Qingdao 266072,China;School of Fishery,Zhejiang Ocean University,Zhoushan 316022,China)
出处
《中国水产科学》
CAS
CSCD
北大核心
2019年第2期342-352,共11页
Journal of Fishery Sciences of China
基金
国家自然科学基金项目(41676153)
农业部物种资源保护费项目(171721301354051028)
关键词
金乌贼
洄游时期
形态差异
微卫星
群体
Sepia esculenta
migratory period
morphological difference
microsatellite
population
