摘要
目的:探讨长链非编码RNA 00707(lncRNA 00707)和微小RNA-613(miR-613)对胃癌细胞增殖和转移的调控作用及其相关机制。方法:收集2014年1月至2018年6月青海省人民医院肿瘤外科89例原发性胃癌组织及癌旁组织标本以及胃癌MGC-803、SGC-790、BGC-823细胞,采用qPCR实验检测胃癌组织和细胞系中lncRNA 00707、miR-613的表达水平。分别建立lncRNA 00707低表达和过表达细胞模型,采用CCK-8实验检测MGC-803、SGC-7901细胞增殖能力,Transwell法检测MGC-803、SGC-7901细胞迁移和侵袭能力。用双荧光素酶基因报告实验验证lncRNA 00707与miR-613的靶向关系。结果:与癌旁组织相比,胃癌组织中lncRNA 00707呈明显高表达(P<0.01),lncRNA 00707表达水平与WHO分期呈正相关(P<0.05);与正常细胞系GES-1相比,胃癌细胞系中lncRNA 00707表达明显上调(P<0.05)。敲低lncRNA 00707可明显抑制SGC-7901细胞的增殖、侵袭和迁移能力(均P<0.05);lncRNA 00707过表达可明显提高MGC-803细胞的增殖和迁移能力(P<0.05)。与阴性对照组相比,lncRNA00707过表达显著降低了miR-613荧光素酶报告载体的荧光素酶活性,而下调MGC-803和SCG-7901细胞中lncRNA 00707,miR-613的表达显著增加(均P<0.01)。结论:lncRNA 00707在胃癌组织和细胞系中发挥促癌效应,其可以通过抑制miR-613而促进胃癌MGC-803和SCG-7901细胞的增殖和转移。
Objective:To investigate the role of long chain non-coding RNA00707(lncRNA 00707)and micro RNA-613(miR-613)in regulating the proliferation and metastasis of gastric cancer cells and its underlying mechanisms.Methods:Eighty-nine pairs of primary gastric cancer tissues and corresponding prar-cancerous tissues were collected from the Department of Surgical Oncology,Qinghai Provincial People’s Hospital during January 2014 and June 2018 for this study.The expressions of lncRNA 00707 and miR-613 in gastric cancer tissues and cells were detected by qPCR.The lncRNA 00707 low expression and over-expression models of MGC-803 and SGC-7901 cells were established;The proliferation of gastric cancer cells was monitored by CCK-8 assay,and Transwell assay was performed to determine the migration and invasion of gastric cancer cells.Dual luciferase gene reporter assay was adopted to validate the relationship between lncRNA00707 and miR-613.Results:Compared with para-cancerous tissues and normal cell line GES-1,the expression of lncRNA 00707 was significantly up-regulated in cancer tissues and cell lines,and the expression of lncRNA 00707 was positively correlated with WHO stage(all P<0.05).Down-regulation of lncRNA 00707 significantly inhibited the proliferation and migration of SGC-7901 cells,while overexpression of lncRNA 00707 exerted the opposite effect(all P<0.05).Compared with negative control group,lncRNA 00707 over-expression significantly reduced the luciferase activity of miR-613;in the contrary,the luciferase activity of miR-163 was significantly increased in MGC-803 and SGC-7901 cells with lncRNA 00707 knockdown(all P<0.01).Conclusion:lncRNA 00707 facilitates the proliferation,migration and invasion of gastric cancer cells by inhibiting the function of miR-613,which exerts a protumorigenic effect in gastric cancer.
作者
吕海栋
周堤侠
祁玉娟
LYU Haidong;ZHOU Dixia;QI Yujuan(Department of Surgical Oncology,Qinghai Provincial People's Hospital,Xining 810007,Qinghai,China;Interinal Medicine-Oncology,Qinghai Provincial People's Hospital,Xining 810007,Qinghai,China)
出处
《中国肿瘤生物治疗杂志》
CAS
CSCD
北大核心
2019年第9期999-1005,共7页
Chinese Journal of Cancer Biotherapy
基金
青海省科技计划资助项目(No.2017-ZJ-907)~~
