摘要
目的 探讨葛根素对臂丛神经根性撕脱伤(brachial plexus root avulsion injury,BPRAI)脊髓前角iNOS、CGRP蛋白表达及PI3K/Akt信号通路的影响。方法 将50只雄性SD大鼠随机分为正常组、模型组、葛根素低、中、高剂量治疗组,每组10只。模型组,葛根素低、中、高剂量治疗组进行BPRAI造模,撕脱大鼠右侧C5~7脊神经前根,后根剪断,术后3个治疗组予腹腔注射葛根素,剂量分别为50、100、200 mg·kg-1·d-1,正常组、模型组腹腔注射等体积生理盐水,持续4周。采用尼氏染色、免疫荧光化学、Western blot方法,观察损伤侧脊髓前角α运动神经元(alpha motorneurons,α-MNs)的存活率,iNOS、CGRP、PI3K/Akt通路相关蛋白的表达。结果 第4周时,低、中、高剂量的葛根素治疗可抑制α-MNs丢失(P<0.05或P<0.01);中、高剂量的葛根素治疗可抑制iNOS表达(P<0.05);高剂量的葛根素治疗可促进CGRP蛋白表达(P<0.05或P<0.01);低、中、高剂量葛根素均可显著抑制p-Akt1/2/3表达(P<0.01)。结论葛根素可改善BPRAI造模引起的α-MNs死亡,其机制可能与葛根素能抑制iNOS蛋白的表达、促进CGRP蛋白的表达有关,并且PI3K/Akt信号通路参与其调控。
Objective To investigate the effect of Puerarin on the expression of iNOS,CGRP and PI3K/Akt signaling pathway in spinal anterior horn following brachial plexus root avulsion injury(BPRAI).Methods Fifty SD rats were randomly divided into a normal group,a model group,a low-dose group,a middle-dose group and a high-dose group with 10 rats in each group.BPRAI modeling was made in the model group,low-dose group,middle-dose group and high-dose group by avulsing the anterior roots and transecting posterior roots of right C5~7 segment of spinal nerve.Puerarin was injected intraperitoneally in the low-dose group,middle-dose group and high-dose group after the operation at doses of 50,100 and 200 mg·kg-1·d-1 respectively.The normal group and model group were given intraperitoneal injection of saline of equal volume for 4 weeks.Nissl staining,immunofluorescence and Western blot were used to observe the survival rate,the expression of iNOS and CGRP of alpha motorneurons(α-MNs),and PI3K/Akt pathway related proteins in the spinal cord on the injured side.Results At the 4th week,low,middle and high doses of Puerarin could inhibit the loss ofα-MNs(P<0.05 or P<0.01);middle and high doses of Puerarin could inhibit the expression of iNOS(P<0.05);high doses of Puerarin could significantly promote the expression of CGRP protein(P<0.05 or P<0.01);low,middle and high doses of Puerarin could significantly inhibit the expression of p-Akt1/2/3(P<0.01).Conclusions Puerarin can ameliorate the death of alpha-MNs induced by BPRAI.The protective effect of Puerarin may be related to its inhibition of iNOS expression and promotion of CGRP expression,and PI3K/Akt signaling pathway is involved in its regulation.
作者
王雨
陈传奇
王文晟
喻保军
张义
李丹
陈龙菊
WANG Yu;CHEN Chuan-qi;WANG Wen-sheng;YU Bao-jun;ZHANG Yi;LI Dan;CHEN Long-ju(Department of Anatomy,Health Science Center,Hubei Minzu University,Enshi 445000,Hubei Province,China;Department of Obstetrics and Gynecology the Central Hospital of Tujia and Miao Autonomous Prefectur,Hubei Minzu University,Enshi 445000,Hubei Province,China)
出处
《中国临床解剖学杂志》
CSCD
北大核心
2019年第5期517-522,共6页
Chinese Journal of Clinical Anatomy
基金
国家自然科学基金(81260192)
湖北民族大学博士科研项目(MY2018B026)
