摘要
目的研究白介素1受体辅助蛋白(IL-1RAP)在胶质瘤中的作用及其与转录活化因子3(STAT3)的关系。方法构建IL-1RAP表达载体,转染胶质瘤细胞系U251,利用流式±细胞仪检测转染IL-1RAP对U251细胞周期及凋亡影响。利用免疫共沉淀钓取STAT3,检测转染IL-1RAP后STAT3的表达情况,并利用免疫荧光方法检测IL-1RAP与STAT3的共定位情况。结果 IL-1RAP蛋白定位于胶质瘤细胞核。与转染空质粒对照组相比,IL-1RAP有明显的促进肿瘤细胞凋亡[(52.10±5.51)%vs.(7.57±0.54)%,P<0.05]和周期阻滞作用[(68.22±1.96)%vs.(38.31±7.22)%,P<0.05]。通过免疫共沉淀和共定位证实IL-1RAP和STAT3可以相互作用。结论 IL-1RAP有抑制胶质瘤细胞增殖、促进细胞凋亡的作用。IL-1RAP可能通过与STAT3相互作用进入细胞核发挥促进细胞凋亡和抑制细胞周期的作用。
Objective To clarify the biological function of IL-1RAP in glioma cells and its relationship with signal transducer and activator of transcription 3(STAT3). Methods The glioma cell line U251 was transfected with IL-1RAP expression vector. Cell cycle and apoptosis of IL-1RAP-transfected U251 cells were analyzed by flow cytometry. STAT3 expression was analyzed by immunocytochemistry and co-immunoprecipitation. Colocalization of IL-1RAP and STAT3 was detected by immunofluorescence. Results IL-1RAP protein was localized in the nucleus of glioma cells.Compared with control group transfected with empty plasmid, IL-1RAP transfection caused the apoptosis[(52.10±5.51)% vs.(7.57±0.54)%, P<0.05] and cell cycle arrest of U251 cells[(68.22±1.96)% vs.(38.31±7.22)%, P<0.05]. In addition, IL-1RAP and STAT3 were colocalized and may interacted to affect the prognosis of glioma cells. Conclusion IL-1RAP could inhibit the proliferation and promote the apoptosis of glioma cells, which may be related with its interaction with STAT3.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2015年第1期23-27,共5页
Cancer Research on Prevention and Treatment
基金
国家自然科学基金(81072074
31271119)
关键词
白介素1受体辅助蛋白
转录活化因子3
胶质瘤
细胞周期
凋亡
Interleukin 1 receptor accessory protein(IL-1RAP)
Signal transducer and activator of transcription 3(STAT3)
Gliomas
Cell cycle
Apoptosis
