摘要
目的:探讨尿路上皮癌抗原1(urothelial carcinoma antigen 1,UCA1)在胰腺癌细胞中的表达及其对胰腺癌PANC-1细胞系侵袭转移的影响。方法:用荧光定量PCR法检测4种胰腺癌细胞系中UCA1的表达水平,选取PANC-1细胞系,将其随机分为对照组和实验组,对照组细胞转染空载Vector,实验组细胞转染UCA1表达质粒,比较两组细胞UCA1表达水平;Transwell迁移实验和Transwell侵袭实验分别检测两组细胞迁移和侵袭能力;蛋白质印迹检测两组细胞MMP14、MMP2和MMP9蛋白表达。结果:UCA1差异性表达于4种胰腺癌细胞系中;与对照组相比,实验组细胞的迁移和侵袭能力明显增强(P<0.01),细胞中MMP14、MMP2和MMP9的蛋白表达水平均明显增加(P<0.01)。结论:上调UCA1促进胰腺癌PANC-1细胞的体外侵袭转移。
Objective: To investigate the expression of urothelial carcinoma antigen 1( UCA1) in pancreatic cancer cell lines and its influence on the invasion and metastasis of the pancreatic cancer cell line PANC-1. Methods: The expression level of UCA1 in 4 pancreatic cancer cell lines was detected by realtime PCR. PANC-1 cells were randomly divided into Vector group and UCA1 group,which was transfected with Vector and UCA1-plasmid,respectively; the UCA1 expression level in two groups was compared. The ability of migration and invasion in vitro in Vector group and UCA1 group were measured by Transwell migration assay and Transwell invasion assay. The protein level of MMP14,MMP2 and MMP9 in two groups was analysed by Western blotting. Results: UCA1 differentially expressed in 4 pancreatic cancer cell lines.Compared with the Vector group,the ability of migration and invasion in UCA1 group were dramatically enhanced( P < 0. 01),and the protein level of MMP14,MMP2 and MMP9 in PANC-1 were increased( P <0. 01). Conclusion: Up-regulation of UCA1 promotes the invasion and metastasis in vitro of PANC-1.
出处
《江苏大学学报（医学版）》
CAS
2015年第4期304-307,共4页
Journal of Jiangsu University：Medicine Edition
基金
江苏省卫生厅科研资助项目(H201434)
镇江市科技支撑计划资助项目(SH2014024)
关键词
胰腺癌
肿瘤转移
长链非编码RNA
尿路上皮癌抗原1
pancreatic cancer
tumor metastasis
long non-coding RNA
urothelial carcinoma antigen 1
