摘要
目的:观察双酚A对雄性小鼠睾丸和附睾中兰尼碱受体(ryanodine receptors,RyRs)基因表达的影响及雌激素受体(estrogen receptor,ERα和ERβ)在其中的作用。方法:将野生型小鼠、ERα基因敲除小鼠(αERKO)和ERβ基因敲除小鼠(βERKO)均随机分为双酚A组[100μg/(kg·d)]和对照组,经口灌胃染毒30 d后(对照组以玉米油灌胃),荧光定量PCR法检测睾丸和附睾中RyR1,RyR2和RyR3 mRNA表达的变化。结果:双酚A可使小鼠睾丸中RyR1和RyR3 mRNA表达水平均明显升高,而RyR2 mRNA表达水平显著降低,附睾中RyR1、RyR2和RyR3 mRNA表达水平明显升高(P均<0.05)。应用ERKO小鼠实验结果显示,双酚A通过ERα和ERβ影响睾丸RyR2以及附睾RyR2和RyR3 mRNA的表达,对附睾RyR1 mRNA的调控则依赖于ERα。结论:双酚A可以影响雄性小鼠睾丸和附睾中兰尼碱受体3种亚型的基因表达,ERα和ERβ在此过程中发挥了重要作用。
Objective: To explore the possible effects of bisphenol A on ryanodine receptors( RyRs) in mouse testis and epididymis,the role of the two ERs was also investigated. Methods: Adult male wild type( WT),ERs knock out mice( αERKO and βERKO) were randomly divided into control and bisphenol A[100 μg /( kg·d) for 30 days via intragastric administration]treatment groups. Quantitative real time PCR was used to examine RyR1,RyR2 and RyR3 genes of the testis and epididymis. Results: The mRNA expression of RyR1 and RyR3 were increased in bisphenol A-treated animals,however,RyR2 was evidently decreased. In the epididymis,the mRNA expression of RyR1,RyR2 and RyR3 were significantly increased( P <0. 05). Bisphenol A up-regulation of RyR1 of the epididymis mRNA expression was dependent on ERα,whereas bisphenol A regulation of RyR2 of the testis and RyR2,RyR3 of the epididymis appeared to be dependent on both ERα and ERβ. Conclusion: The ERs signalling was necessary for the regulation of the RyR channels mRNA expression by bisphenol A,which could affect the male reproductive function.
出处
《江苏大学学报（医学版）》
CAS
2015年第1期1-4,共4页
Journal of Jiangsu University：Medicine Edition
基金
国家自然科学基金资助项目(81400911)
江苏省自然科学基金资助项目(BK20140573)
中国博士后科学基金资助项目(2014M560407)
江苏大学高级人才基金资助项目(12JDG083)
江苏省博士后科研基金资助项目(1301045B)
现代毒理学教育部重点实验室(南京医科大学)开放基金资助项目(NMUMT201402)
