摘要
利用UPLC-Q-TOF/MS^E结合主成分分析(PCA)及正交偏最小二乘判别分析(OPLS-DA),研究了钩吻老茎和嫩茎生物碱类成分的差异,找出了17个主要的差异性成分,并明确了差异性生物碱在老茎及嫩茎中的分布,其中极性较大的生物碱在钩吻嫩茎中含量较高,而在老茎中,极性较小的生物碱含量较高。同时利用HPLC-UV定量分析了钩吻中3个主要的生物碱。结果表明老茎中的钩吻素子和钩吻素己含量高于嫩茎,而嫩茎中钩吻素甲含量较高。该研究系统分析了钩吻老茎及嫩茎生物碱类成分的组成及差异,同时对其3个主要的生物碱成分进行了定量分析,为钩吻药材安全有效的应用奠定了基础。
This study aimed to comprehensively assess the difference of alkaloid components between old stems and tender stems of Gelsemium elegans by using ultra high-performance liquid chromatography coupled with photo-diode array and quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF/MS^E)and high-performance liquid chromatography coupled with UV detector(HPLC-UV).Firstly,the different components in old stems and tender stems were analyzed by UHPLC-Q-TOF/MSEcombined with principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA),respectively.As a result,17 major different components were found.At the same time,the distribution of these alkaloids in old stems and tender stems was determined,and the alkaloids with higher polarity are relatively higher in the tender stems,while the old stems are in the opposite case.In addition,three main components in the G.elegans were quantified by HPLC-UV.The results showed that the contents of koumine and humantenmine in old stems were higher than those in tender stems,and the content of gelsemine in tender stems was relatively high.This study systematically evaluated the differences of alkaloids between the old stems and tender stems of G.elegans,and quantified the main three alkaloids.It laid the foundation of the safe and effective application of G.elegans.
作者
王杰
张洁
张春泥
廖晓春
李锦
马百平
WANG Jie;ZHANG Jie;ZHANG Chun-ni;LIAO Xiao-chun;LI Jin;MA Bai-ping(Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China;Institute of Radiation Medicine,Academy of Military Medical Sciences,Academy of Military Sciences,Beijing 100850,China;Yunnan Mingjinghengli Pharmaceutical Co.,Ltd.,Kunming 650100,China;Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences,Academy of Military Sciences,Beijing 100850,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2019年第12期2552-2558,共7页
China Journal of Chinese Materia Medica
关键词
钩吻
老茎
嫩茎
生物碱
差异
Gelsemium elegans
old stems
tender stems
alkaloids
difference
