摘要
目的探讨钩吻(GelsemiumelegansBenth)提取液对Hela细胞生长增殖以及细胞周期的影响。方法采用XTT法分析细胞增殖,Timelapse显微镜形态观察,流式细胞仪检测凋亡和细胞增殖状态,综合评价钩吻提取液抗Hela细胞生长和增殖的作用。结果钩吻B组分具有明显的抑制Hela细胞增殖和诱导凋亡,且存在明显的剂量和时间-效应关系。经钩吻B处理的Hela细胞周期发生明显变化,G0/G1期细胞比例降低,S期细胞比例增加,而G2/M期细胞比例变化不明显。结论钩吻B组分可抑制Hela细胞增殖、诱导凋亡,具有明显的抗肿瘤细胞的作用;钩吻B组分主要阻止细胞由G1期向S期转化,并在此阶段诱发凋亡。
To investigate the effects of Gelsemium elegans Extract on the proliferation of Hela Cell growth and cell cycle. Analysing cell proliferation in the way of XTT and watching it under a Tinelapse Microscope. Testing the apoptosis and proliferation state by using fluo-cytometry. Comprehensive evaluation the effects of Gelsemium elegans Extract on Hela Cell growth and its proliferation. Group B in Gelsemium was obvious. Remarkable retardation on cell proliferation and apoptosis was observed with Gelsemium treated sample (Group B). The effect was dose and time dependent. The cell cycle in Group B has changed significantly. The G0/G1 ratio of cultured cell was declined and cells in S phase was founded increased. The proportion of S period cell has risen. But there was not any obvious change in the proportion of G2/M phase cell. [Conclusion] Gelsemium elegans Extract can prohibit the prolification of Hela Cell and induce apoptosis. Antitumor effect of Gelsemium elegans Extract is obviously observed. Thus Group B in Gelsemium may prevent the cell transiton form G1 phase to S Phase and induce apoptosis during this period.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2005年第2期230-232,共3页
China Journal of Modern Medicine
基金
西班牙-中国国际科技合作项目(2002CN0008)
