摘要
为挖掘非酒精性脂肪肝的组方用药规律,分析处方可能存在的药物靶标及相互作用,探寻药物潜在作用机制。收集中国知网、万方、维普、中国生物数据库和PubMed等数据库中关于中药治疗非酒精性脂肪肝的随机对照试验;运用SPSS 19. 0软件对药物的性味归经进行系统聚类分析;采用Clementine 12. 0软件对数据进行关联规则分析;运用TCMSP,String,KEGG数据库分析常用药物靶标间相互作用及相关通路。筛选出88首处方,挖掘出常用药物为丹参、柴胡、泽泻、山楂,与非酒精性脂肪肝关系密切的潜在通路是PPAR信号通路和钙离子通路。结果表明,中药治疗非酒精性脂肪肝以活血化瘀、清热祛湿、消食健脾为主,其作用机制可能通过PPAR信号通路和钙离子通路实现。
To explore the medication rules of herbal prescriptions for nonalcoholic fatty liver disease,and analyze the possible drug targets and interactions,in order to explore the mechanisms of the herbs.Randomized controlled trials of herbal prescriptions for treating nonalcoholic fatty liver disease were collected from CNKI,Wan Fang,VIP,Sino Med and PubMed databases.The properties,flavors and meridian tropism of herbs were analyzed by using systematic cluster analysis method with SPSS 19.0 software.Subsequently,the association rules of herbs were analyzed by using Clementine 12.0 software.Finally,the interactions between targets and relevant signaling pathways were analyzed by Traditional Chinese Medicine Systems Pharmacology Database(TCMSP),Search Tool for the Retrieval of Interacting Genes/Proteins(STRING)and Kyoto Encyclopedia of Genes and Genomes(KEGG).In the 88 prescriptions screened out,the commonly used herbs were Salvia miltiorrhiza,Bupleurum chinense,Alisma orientale,and Crataegus pinnatifida,and the potential signaling pathways were PPAR signaling pathway and calcium signaling pathway.The results showed that the main effects of herbal prescriptions were to improve blood flow/clear blood stasis,clear heatiness/dampness,promote digestion and strengthen spleen.And its mechanism of action may be achieved through the regulation of PPAR signaling pathway and calcium signaling pathway.
作者
丁静
张斌
王培劼
何国浓
魏冬梅
丁佳璐
王邦才
DING Jing;ZHANG Bin;WANG Pei-ji;HE Guo-nong;WEI Dong-mei;DING Jia-lu;WANG Bang-cai(Department of Gastroenterology,Ningbo Hospital of Traditional Chinese Medicine,Ningbo 315012,China;Science and Education Department,Ningbo Hospital of Traditional Chinese Medicine,Ningbo 315012,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2019年第8期1689-1695,共7页
China Journal of Chinese Materia Medica
基金
浙江省名老中医专家传承工作室建设计划项目(GZS2017018)
宁波市自然基金项目(2018A610423)
关键词
非酒精性脂肪肝
数据挖掘
生物信息
用药规律
作用机制
nonalcoholic fatty liver disease
data mining
biological information
medication rules of herbal prescriptions
mechanisms of herbal prescriptions
