摘要
亲和色谱具有特异性强、回收率高和灵敏便捷的特点,广泛应用于中药活性成分的筛选、低分子糖和蛋白多肽的分离富集、作用机制研究及作用靶点的发现。该文综述了亲和色谱及其吸附等温模型、动力学模型和吸附热力学机制在中药领域的应用,为中药活性成分筛选、分离纯化和药物相互作用领域中亲和色谱吸附理论的应用提供借鉴和参考。
Affinity chromatography is characterized by its high specificity,high recovery rate and sensitivity,and it has been widely used in the selection of active ingredients of traditional Chinese medicine,separation and enrichment of low molecular weight sugars and protein peptides,research on mechanism of action and discovery of targets.This paper reviewed the application of affinity chromatography and its adsorption isotherm model,kinetic model and adsorption thermodynamic mechanism in the field of traditional Chinese medicine.This summarizes and provides thinking for comprehensive applications of affinity chromatography theory in the field of active ingredient screening,purification and medicine interaction.
作者
陈昱初
赵露
徐希明
余江南
童珊珊
CHEN Yu-chu;ZHAO Lu;XU Xi-ming;YU Jiang-nan;TONG Shan-shan(School of Pharmacy,Jiangsu University,Zhenjiang 212013,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2019年第1期40-47,共8页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81673574)
中国博士后科学基金面上项目(2014M560409)
关键词
亲和色谱
中药
吸附等温模型
动力学模型
吸附热力学
affinity chromatography
traditional Chinese medicine
adsorption isotherm model
kinetic model
adsorption thermodynamics
