摘要
为确定河北省某牛场牛细菌病的病原,本研究从采集的发病牛鼻腔拭子样品中分离到1株细菌,并对其进行常规形态学观察、生化鉴定、16S rDNA基因序列测定、动物致病性试验及药物敏感试验。结果显示,该分离菌株与腔隙莫拉菌的同源性为97.6%,系统发育进化分析显示其与腔隙莫拉菌位于同一进化分支;该菌株对小白鼠具有一定的致病性,将其命名为Mor QHD-1。西药药敏试验结果显示,分离菌株Mor QHD-1对氨苄西林、环丙沙星、阿米卡星等敏感;对磺胺间甲氧嘧啶、复方新诺明、磺胺二甲氧嘧啶耐药;中药药敏试验结果显示,分离菌株Mor QHD-1对苏木、诃子、乌梅、黄柏、五味子、黄连、五倍子极敏感;苏木、诃子、乌梅、黄柏、五味子、黄连、五倍子对该菌的最小抑菌浓度在1.95 mg/mL～31.25 mg/mL之间,最小杀菌浓度在3.91 mg/m L～62.5 mg/mL之间;本研究在国内首次分离到牛源腔隙莫拉菌,为进一步研究腔隙莫拉菌的致病机制、耐药机制及其跨物种传播奠定了基础。
In order to identify the bacteroidal pathogen from the affected cattle in a cattle farm in Hebei province,a dominant bacteria was isolated from nasal cavity of sick cattle and identified as Moraxella lacunata by using conventional morphological observation,biochemical tests,bacteria 16 S rDNA gene sequencing,pathogenicity test and drug sensitive test,demonstrating that the isolate 16 S rDNA was 97.6%homologous to M.lacunata,named Mor QHD-1,which had certain pathogenicity to mice.The drug susceptibility tests showed that the isolate of Mor QHD-1 was sensitive to ampicillin,ciprofloxacin,amikacin,etc.but resistance to sulfamonomethoxine,compound sulfamethoxazole and sulfamethoxazole.The results of traditional Chinese medicine susceptibility test showed that the isolate of Mor QHD-1 was extremely sensitive to Caesalpinia sappan,Terminalia chebuLa,Prunus mume,Phellodendron amurense,Schisandra chinensis,Coptis chinensis and Galla chinensis;The minimum inhibitory concentration of Caesalpinia sappan,Terminalia chebuLa,Prunus mume,Phellodendron amurense,Schisandra chinensis,Coptis chinensis and Galla chinensis was ranged from 1.95 mg/mL to 31.25 mg/mL and the minimum bactericidal concentration of Caesalpinia sappan,Terminalia chebuLa,Prunus mume,Phellodendron amurense,Schisandra chinensis,Coptis chinensis and Galla chinensis was ranged from 3.91 mg/mL to 62.5 mg/m L.In this study,a bovine M.lacunata was isolated for the first time in China,and the M.lacunata of Mor QHD-1 would facilitate further study of the pathogen mechanism,resistance mechanism and cross species transmission.
作者
朱利霞
王洪彬
赵希艳
王双月
史秋梅
高光平
ZHU Li-xia;WANG Hong-bin;ZHAO Xi-yan;WANG Shuang-yue;SHI Qiu-mei;GAO Guang-ping(Hebei key laboratory of preventive veterinary medicine,Hebei Normal University of Science&Technology,Qinhuangdao 066600,China)
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2019年第3期251-255,共5页
Chinese Journal of Preventive Veterinary Medicine
基金
河北省现代农业产业体系肉牛产业创新团队疫病防控岗(HBCT2018130203)
河北省教育厅(SLRC2017037)
河北省高层次人才资助项目(A2016005046)
河北科技师范学院科学研究基金项目
关键词
牛
腔隙莫拉菌
分离与鉴定
药敏试验
cattle
Moraxella lacunata
isolation and identification
drug susceptibility test
