摘要
以温敏型核雄性不育系T-4及对照First为材料,采用考马斯亮蓝G-250染色通过分光光度法测定花药蛋白质含量,通过聚丙烯酰胺凝胶垂直板电泳分析POD和EST同工酶的酶谱差异。试验结果表明,可育温度条件下的T-4花药蛋白质含量显著高于不育温度处理,但与对照First差异不显著。POD及EST同工酶酶谱显示,在不育温度条件下T-4的POD及EST同工酶谱带均多于可育温度处理及对照First;可育温度条件下T-4的POD及EST同工酶谱带与对照First差别不明显。
The experimental materials were thermo-sensitive genic male-sterile tomato(Lycopersicum esculentum Mill.)T-4 and the control First.The protein content was determined by spectrophotometry,adopting Coomassiebrilliant blue G250 as dye.Meanwhile,the activity of POD and EST isozyme was analyzed with the polyacrylamide gel electrophoresis of perpendicular plate.Results showed that the protein content of T-4 anther under fertile condition was significantly higher than that under sterile condition,but was no significant difference with the control First.The band of POD and EST isozyme in anther of T-4 under sterile condition was more than that in anther of T-4 under fertile condition,and the control First.There was no significant difference in band of POD and EST isozyme between T-4 and the control First,when T-4 was under fertile situation.
出处
《中国蔬菜》
北大核心
2013年第03X期58-62,共5页
China Vegetables
基金
国家科技部国际合作项目(2006DFA33380)
广西科技厅广西科学研究与技术开发计划项目(0718007B-38)
南宁市青秀区科学技术局科学研究与技术开发计划项目(20080211B)
桂林市局科学研究与技术开发计划(20100112-1)
现代农业产业技术体系建设专项资金(CARS-25)
