摘要
含有ps-2雄性不育基因的番茄材料可通过自交获得100%的雄性不育后代,从而方便地应用于番茄杂交制种。以优良的加工番茄品系92155为父本,以来自保加利亚含有ps-2基因的不育材料CMC1ps2为母本,构建了123个F2代分离群体,育性表型分离比例完全符合孟德尔遗传规律,为隐性单基因。根据番茄形态和分子标记整合遗传连锁图谱,选取相应的SSR、RFLP及COS标记,分别筛选了5对SSR引物、13对由RFLP探针序列转化来的CAPS引物及1对COSⅡ引物。经连锁遗传分析,获得了与ps-2基因紧密连锁的1个SSR标记(SSR450)和1个CAPS标记(命名为TG123),它们与ps-2基因的连锁遗传距离分别是4.9cM和11.6cM,位于该基因两侧,均为共显性标记。这两个标记的获得可直接用于标记辅助育种和杂种纯度鉴定,加速番茄雄性不育的转育与利用。
Tomatoes with ps-2 gene can produce the progeny with 100% male sterility through selfing hence it is easy to be used for hybrid production.An elite processing tomato inbred line 92155 as male parent and a line named as CMC1ps2 with ps-2 gene from Bulgaria as female parent were employed to produce a F2 segregation population with 123 individuals.The segregation ratio of sterility was consistent with the Mendel law as a single recessive gene.Based on the maps of morphological and molecular marker,SSR,RFLP and COS markers were selected corresponding to ps-2 locus.In total,5 SSR markers,13 CAPS markers proved developed from RFLP probe sequences and one COS marker were screened.The linkage analysis that one SSR marker （SSR450） and one CAPS marker （TG123） were tightly linked with ps-2 gene.The linkage distances were about 4.9 and 11.6 cM respectively and both presented co-dominant character.Markers obtained can be directly used for marker assisted selection and the identification of hybrid purity.It also would speed up the procedure for the introgression and application of tomato male sterility in breeding.
出处
《园艺学报》
CAS
CSCD
北大核心
2010年第2期235-240,共6页
Acta Horticulturae Sinica
基金
农业部园艺作物遗传改良重点开放实验室项目
