摘要
目的基于血清药物化学方法,研究血栓心脉宁片ig给予病理大鼠后的入脑移行成分。方法通过冰水浴-肾上腺素法复制急性血瘀大鼠模型,并ig给予血栓心脉宁片[1.4 g/(kg·d)] 8 d,末次ig给药后收集脑组织样本,继而采用超高效液相色谱-四级杆飞行时间质谱联用(UPLC-Q-TOF/MS)技术与多元统计分析相结合的方法快速分析并鉴定吸收入脑的原型成分及其代谢产物。结果从血瘀大鼠脑组织中鉴定出包括菲醌和蟾酥甾二烯等其他结构类型的11种原型入脑成分及3个代谢产物。结论 UPLC-Q-TOF/MS分析方法的建立全面阐释了血栓心脉宁片的入脑原型成分及其代谢产物,为该中药大品种的药效物质基础研究提供了科学依据。
Objective To study the constituents and metabolites in rat brain after ig administration of Xueshuan Xinmaining Tablet(XXT)based on the serum medicinal chemistry.Methods The acute blood stasis rat model was replicated by ice bath-adrenalin method,and XXT[1.4(g·kg-1·d-1)]was administered orally for 8 d.The brain samples were collected and pretreated after ig administration for the last time.Then the absorbed prototype constituents and their metabolites in rats plasma were rapidly analyzed and identified by combination of ultra-high performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry(UPLC-Q-TOF/MS)and multivariate statistical analysis.Results Finally,a total of 11 absorbed prototype constituents and three metabolites were identified,including phenanthrene,bufonis venenum oxadiene,and other structural types of compounds.These constituents absorbed into brain may be the potential bioactive components in XXT.Conclusion The establishment of UPLC-Q-TOF/MS analysis method explained comprehensively the brain migration component of XXT,and provided scientific basis for clarifying its substance basis of pharmacodynamics of this large variety of Chinese medicine.
作者
谭静
林红强
刘云鹤
王涵
董庆海
吴福林
朱继忠
刘传贵
李平亚
刘金平
朱海林
TAN Jing;LIN Hong-qiang;LIU Yun-he;WANG Han;DONG Qing-hai;WU Fu-lin;ZHU Ji-zhong;LIU Chuan-gui;LI Ping-ya;LIU Jin-ping;ZHU Hai-lin(Natural Product Research Center,College of Pharmacy,Jilin University,Changchun 130021,China;Jilin Huakang Pharmaceutical Co.,Ltd.,Dunhua 133700,China)
出处
《中草药》
CAS
CSCD
北大核心
2019年第17期4254-4260,共7页
Chinese Traditional and Herbal Drugs
基金
吉林省省校共建中药专项项目(SXGJSF2017-1-1)
关键词
血栓心脉宁片
UPLC-Q-TOF/MS
入脑成分
血清药物化学
菲醌
蟾酥甾二烯
Xueshuan Xinmaining Tablet
UPLC-Q-TOF/MS
absorbed constituents in brain
serum medicinal chemistry
phenanthrene
bufonis venenum oxadiene
