摘要
基于血清药物化学方法,研究血栓心脉宁片(XXT)灌胃给予血瘀大鼠的血中移行成分。通过冰水浴-肾上腺素法复制急性血瘀大鼠模型,并灌胃给予XXT〔1.4 g/(kg·d)〕8 d,末次给药后收集血清样本,继而采用超高效液相色谱-飞行时间质谱联用(UPLCQ/TOF-MS)技术,结合多元统计分析,鉴定XXT的血中移行成分。从血瘀大鼠血清中鉴定出包括菲醌、三萜皂苷及甾体等结构类型的28个原型入血成分及3个代谢产物。UPLC-Q-TOF/MS分析方法的建立,对XXT的血中移行成分进行了全面阐释,为该中药大品种的药效物质基础研究奠定了基础。
Based on the serum medicinal method,the blood-transition constituents in rats serum after oral administration of Xueshuan Xinmaining Tablet(XXT)were quickly and comprehensively analyzed and identified.The acute blood stasis rat model was replicated by ice bath-adrenalin method,and XXT〔1.4 g/kg·d〕was administered orally for 8 days.The serum samples were collected and pretreated after oral administration for the last time.Then,the absorbed prototype constituents and their metabolites in rats plasma were rapidly analyzed and identified by UPLC-Q-TOF/MS,an ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry,coupled with multivariate statistical analysis.Finally,a total of 28 absorbed prototype constituents and 3 metabolites were identified,including phenanthrene,triterpenoid saponins,steroids and other structural types of compounds.these constituents absorbed into blood may be the potential bioactive components in XXT.The establishment of UPLC-Q-TOF/MS analysis method explained comprehensively the blood migration component of XXT,and provided scientific basis for clarifying its substance basis of pharmacodynamics of this large Chinese medicine.
作者
谭静
林红强
刘云鹤
王涵
董庆海
吴福林
朱继忠
刘传贵
李平亚
刘金平
刘俊丽
TAN Jing;LIN Hong-qiang;LIU Yun-he;WANG Han;DONG Qing-hai;WU Fu-lin;ZHU Ji-zhong;LIU Chuan-gui;LI Ping-ya;LIU Jin-ping;LIU Jun-li(Natural Product Research Center,College of Pharmacy,Jilin University,Changchun 130021,China;Jilin Huakang Pharmaceutical Company Limited,Dunhua 133700,China)
出处
《特产研究》
2019年第3期89-96,共8页
Special Wild Economic Animal and Plant Research
基金
吉林省省校共建中药专项项目（SXGJSF2017-1-1）
