摘要
目的 :研究白细胞介素 2 (IL 2 )对人乳腺癌Bcap 37细胞系增殖的影响 ,并探讨其可能的机制。方法 :以培养的Bcap 37细胞进行了以下实验 :用MTT比色法 ,测定IL 2对该细胞增殖的剂量效应 ;用免疫组化染色法 ,检测其表面IL 2的表达 ;用RT PCR技术 ,检测该细胞中IL 2Rα、β和γmRNA的表达 ;用流式细胞术 ,观察IL 2对该细胞细胞周期各阶段DNA含量的影响 ;用激光扫描共聚焦显微镜技术 ,检测IL 2对该细胞内Ca2 + 水平的影响。结果 :IL 2 (1× 10 5～ 1× 10 6U/L)可显著促进乳腺癌细胞Bcap 37的增殖。培养的Bcap 37细胞可分泌IL 2并表达IL 2Rα、β和γ。用流式细胞仪检测Bcap 37细胞中DNA的含量发现 ,IL 2 (1× 10 5U/L)可提高该细胞由G1期进入G2 和S期的比例。激光共聚焦法检测到IL 2 (1× 10 5U/L)可使Bcap 37细胞内的Ca2 + 浓度降低。结论 :IL 2参与了对乳腺癌细胞增殖的调节 ,其促增殖作用与该细胞上IL 2受体的表达 ,细胞中DNA含量的变化和Ca2 + 浓度的降低有关。
AIM: To investigate the effects of IL 2 on the proliferation of human mammary carcinoma cell line Bcap 37 and explore its possible mechanism. METHODS: Enhancement effect of IL 2 on proliferation of cultured Bcap 37 cells, the IL 2 expression on the cells, the expressions of IL 2Rα, β, γ mRNAs in the cells, the effect of IL 2 on DNA content at various periods of cell cycle and on Ca 2+ concentration in the cells were detected or observed by MTT colorimetry, immunohistochemical staining, RT PCR, flow cytometry and laser scanning confocal microscope, respectively. RESULTS: IL 2 of 1×10 5 to 1×10 6 U/L significantly stimulated the proliferation of cultured Bcap 37 cells. IL 2 was secreted and IL 2Rα, β and γ were expressed by cultured Bcap 37 cells. IL 2(1×10 5 U/L) increased ratio of Bcap 37 cells in G 2 and S phases and decreased the Ca 2+ release from Bcap 37 cells. CONCLUSION: IL 2 significantly enhance the proliferation of mammary carcinoma cell line Bcap 37. The enhancement effect of IL 2 on Bcap 37 cell proliferation is possibly related to the expression of IL 2R and decreased Ca 2+ concentration in the cells.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2003年第4期390-392,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金资助项目 (No .39870 31 9)
关键词
白细胞介素—2
乳腺癌
细胞增殖
interleukin 2
mammary carcinoma
cell proliferation
