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金黄色葡萄球菌黏附到Bcap-37上皮细胞促进TLR4的表达 被引量:1

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摘要 目的:研究金黄色葡萄球菌对乳腺上皮细胞的黏附及Toll样受体4(TLR4)表达的影响。方法:用不同浓度的金黄色葡萄球菌作用Bcap-37细胞,用平板活菌计数观察金黄色葡萄球菌对Bcap-37细胞的黏附作用;RT-PCR法检测金黄色葡萄球菌作用Bcap-37细胞后TLR4 mRNA的表达变化;流式细胞术分析Bcap-37细胞表面TLR4的表达变化。结果:金黄色葡萄球菌黏附乳腺上皮细胞具有显著的时间和剂量依赖性(P<0.01),作用60 min达到饱和黏附后不再增加。金黄色葡萄球菌作用Bcap-37细胞后,TLR 4 mRNA和蛋白表达量随着作用时间延长而逐渐增高,分别在4 h和6 h达到高峰之后又逐渐下降(P<0.05)。结论:金黄色葡萄球菌能够黏附乳腺上皮细胞,并且可以上调Bcap-37细胞TLR4 mRNA和蛋白的表达。
出处 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2012年第12期1276-1278,共3页 Chinese Journal of Cellular and Molecular Immunology
基金 新疆自治区自然科学基金面上项目(2010211A03)
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