摘要
目的 观察胡椒碱对新西兰兔胆囊结石形成的预防作用和机制。方法 (1)动物模型 :新西兰兔 30只 ,随机分成 :对照组 (10只 ) ,喂普通饮食 ;结石组 (10只 ) ,喂含 1 2 g/d胆固醇的成石饮食 4周 ,胡椒碱组 (10只 ) ,同时喂饲含 1 2 g/d胆固醇和 0 0 4 5 g/d的胡椒碱饮食 4周。 (2 )氨肽酶N(APN)表达 :利用RT PCR方法 ,检测肝APNmRNA水平的变化。 (3)APN活性 :用化学法检测。结果 结石组 6只 (6 / 10 )出现胆囊结石 ,9只 (9/ 10 )出现胆固醇结晶 ,胡椒碱组和对照组无结石及结晶出现 ;结石组APNmRNAIOD比值 (对照组 0 6 5± 0 18,结石组 1 0 8± 0 35 ,胡椒碱组 0 87±0 4 4 ) ,APN活性 (对照组 6 1± 2 5 3U/L、结石组 2 3 6 4± 10 36U/L ,胡椒碱组 8 31± 6 4 7U/L)明显高于对照组和胡椒碱组。结论 以上结果表明胡椒碱主要通过降低肝APN的表达及胆汁APN酶的活性 ,抑制APN的促成石作用而预防胆固醇结石的形成。
Objective To investigate the inhibitory effects of piperine on experimental gallstone formation and explore its mechanism. Methods Three dietary groups of New Zealand rabbits with 10 in each were allocated piperine prevention (1 2 g/d high cholesterol diet + 0 045 g/d piperine) in each group for 4 weeks. RT PCR was performed for APN gene expression in liver tissue. The enzymatic activity of APN in bile was chemically analyzed. Results Comparing with the negative results both in control and piperine prevention groups, cholesterol crystals and stones were found in 9 and 6 out of 10 gallbladders, respectively, under the polarizing microscope in lithogenic group. The integrated opticasl density (IOD) ratio value of mRNA for APN gene was much higher in lithogenic group (1 08±0 35) than that of control and piperine prevention groups (0 65±0 18 and 0 87±0 44 respectively). The enzymatic activity of APN was 6 1±2 53 U/L, 8 31±6 47 U/L and 23 6±10 36 U/L in control, piperine prevention and lithogenic group respectively. Conclusions Piperine may prevent the formation of cholesterol gallstone through inhibiting the lithogenic role of APN.
出处
《中华肝胆外科杂志》
CAS
CSCD
2003年第7期426-428,共3页
Chinese Journal of Hepatobiliary Surgery
