摘要
目的:研究荷叶提取物黄酮对兔胆囊结石形成的影响及机制。方法:45只雄性新西兰兔,随机均分成对照组、结石组和黄酮组,分别喂食普通饮食、含1.2 g/d胆固醇的成石饮食、含1.2 g/d胆固醇和0.4 g/d的黄酮饮食。采用RT-PCR方法检测肝脏氨肽酶N mRNA(APNmRNA)水平的变化,用放射免疫法检测血清亮氨酸氨肽酶(LAP)表达及血脂。结果:结石组10只出现胆囊结石,12只出现胆固醇结晶,黄酮组和对照组无结石及结晶出现;结石组APN mRNA IOD比值(1.08±0.35)明显高于对照组(0.65±0.18)和黄酮组(0.87±0.44);结石组血清总胆固醇、甘油三脂、低密度脂蛋白胆固醇、载脂蛋白B(apoB)与黄酮组相比明显升高(P<0.01),高密度脂蛋白有降低趋势,但无显著性差异(P>0.05);结石组血清载脂蛋白A(apoA)降低(P<0.05);结石组LAP明显高于对照组和黄酮组(P<0.01)。结论:荷叶提取物黄酮可改变血脂水平、降低肝脏APN的表达和血清LAP,从而有助于防止胆囊胆固醇结石的形成。
Objective： To investigate the inhibitory effects of flavone from the lotus on experimental gallstone formation and explore its mechanism. Methods： Forty-five New Zealand rabbits were invided into control group, lithogenic group and flavone prevention group （normal diet, 1.2 g/d cholesterol diet, 1. 2 g/d cholesterol ＋0. 4 g/d flavone diet, respectively）. RT-PCR was performed for APN mRNA expression in liver tissue. The leucine aminopepticlase（LAP） and lipid in blood serum were chemically analyzed. Results： Comparing with the negative results both in control group and flavone prevention group,cholesterol crystals and stones were found in 12 and 10 out of 15 gallbladders, respectively, under the polarizing microscope in lithogenic group. The integrated opticasl density （IOD） ratio value of APN mRNA was much higher in lithogenic group
（1.08±0.35） than that in control and flavone prevention groups （0.65±0.18 and 0.87±0.44, respectively）. TG, TC, LDL-C, LAP and apoB were much higher in lithogenic group than those in control and flavone prevention groups （P〈0.01）. However, apoA was much lower in lithogenic group than that in control and flavone prevention groups （P〈0.05）. Conclusion： Favone may prevent the formation of cholesterol gallstone through inhibiting the lithogenic role of APN.
出处
《武汉大学学报（医学版）》
CAS
2007年第5期573-575,591,共4页
Medical Journal of Wuhan University
基金
湖北省科技攻关重大项目(编号:2002AA205A)
关键词
胆结石
黄酮
氨肽酶N
亮氨酸氨肽酶
Gallstone
Flavone
Aminopeptidase N
Leucine Aminopepticlase
