摘要
Two new triterpenoids, 3 β, 15α, 21 β, 23-tetrahydroxy-12-oleanen-28-oic acid (1), 3β , 6 α , 21β, 23-tetrahydroxy-12-oleanen-28-oic acid (2), together with seven known compounds, viz., paeonol (3), 3, 4-dihydroxybenzoic acid (4), scosoletin (5), anthraquinones chrysophanol (6), 4H-1-benzopyran-4-one, 5, 7-dihydroxy-2-methyl (7),β-sitosterol (8) and stigmasterol glucoside (9) were isolated by the chromatography of the silica gel, RP-18 and Sephadex-LH 20 from the EtOAc extract of Neonauclea sessilifolia (Roxb.) Merr. ( Rubiaceae ). Their structures were elucidated based on spectral analysis including 1D-, 2D-NMR (HMQC, HMBC), IR and EIMS. Among them, compound 6 was shown to possess inhibitory activity on the growth of Mycobacterium tuberculosis (Zopf) Lehmann et Neumann with a minimum inhibitory amount of 25μg, compounds 2 and 4 also showed weak inhibitory activities on the growteof M. tuberculosis.
采用活性跟踪的方法对无柄新乌檀(Neonauclea sessilifolia (Roxb.)Merr.)枝干的乙醇提取物进行结核杆菌活性检测。利用硅胶柱层析、Sephadex-LH 20和反相硅胶RP18柱层析,从具有活性的乙酸乙酯部分分得9个化合物,利用一维、二维核磁共振技术(HMQC,HMBC)并结合质谱与红外光谱,将它们鉴定为3β,15α,21β,23-tetrahydroxy-12-oleanen-28-oic acid(1)、3β,6α,21β,23-tetrahydroxy-12-oleanen-28-oic acid(2)、单皮酚(3)、3,4-二羟基苯甲酸(4)、东莨菪内酯(5)、蒽醌大黄酚(6)、5,7-二羟基-2-甲基吡喃酮(7)、β-谷甾醇(8)和^(5,22)Δ-豆甾醇-β-D-葡萄糖苷(9)。其中化合物1和2是两个新化合物。体外抗结核杆菌试验表明,化合物6对结核杆菌(Mycobacterium tuber-culosis(Zopf)Lehmann et Neumann)具有一定的抑制效果,用量在25μg时出现抑菌圈;化合物2与4具有微弱的抑制活性。
