摘要
目的:研究天疱疮患者血清中抗桥粒芯蛋白(desmoglein,Dsg)1和抗Dsg3抗体水平与其皮肤、口腔黏膜损害严重程度的相关性,同时对间接免疫荧光(IIF)检测的天疱疮抗体滴度与治疗中使用皮质类固醇控制剂量的相关性进行分析。方法:采用酶联免疫吸附试验(ELISA)试剂盒测定55例天疱疮患者血清中抗Dsg1和抗Dsg3抗体水平。结果:抗Dsg1抗体水平与患者皮肤损害严重程度有显著相关性(P<0.01),抗Dsg3抗体水平与口腔黏膜损害严重程度有显著相关性(P<0.01)。天疱疮患者血清IIF滴度与抗Dsg1抗体水平相关(P<0.01),寻常型天疱疮患者IIF滴度与抗Dsg1和抗Dsg3抗体水平均有相关性(P分别<0.01和<0.05)。寻常型天疱疮患者皮质类固醇控制剂量与抗Dsg1抗体水平和IIF滴度显著相关(P<0.05)。结论:ELISA方法检测天疱疮患者抗Dsg1和抗Dsg3抗体对天疱疮的临床诊断、分型、衡量口腔黏膜和皮肤损害严重程度具有一定意义。
Objective: To investigate the correlation between the disease severity and serum level of anti-desmoglein antibodies in patients with pemphigus. Methods: Fifty-five cases of pemphigus were diagnosed based on clinical features, skin biopsy, direct and indirect immunofluorescence. Serum levels of anti-desmoglein-1 (Dsg1) and anti-desmoglein-3 (Dsg3) antibodies in the patients were measured by enzyme-linked immunosorbent assay (ELISA) kits and indirect immunofluorecence on cryostat sections of monkey lips, respectively. Results: Serum levels of anti-Dsg1 antibody were related with the severity of the skin lesions (P < 0.01), while anti-Dsg3 antibody levels were related with the severity of oral involvement (P < 0.01). It was also revealed that IIF titers of pemphigus antibodies were related with the levels of anti-Dsg1 antibody in all 68 serum samples(P < 0.01), however, IIF titers in the patienls with pemphigus vulgaris were significantly related with bolh anti-Dsg1 and anti-Dsg3 antibodies. Furthermore, in palienls wilh pemphigus vulgaris, there was a significant correlation between corticosteroid dosage and the anti-Dsg1 antibodies level. Conclusions: The detection of serum anti-Dsg1 and anli-Dsg3 antibodies by ELISA kits is a useful laboratory method for the diagnosis and monitoring the activity of pemphigus.
出处
《临床皮肤科杂志》
CAS
CSCD
北大核心
2003年第9期512-514,共3页
Journal of Clinical Dermatology
关键词
天疱疮
皮肤损害
黏膜损害
桥粒芯蛋白
酶联免疫吸附试验
pemphigus
skin lesions
mucous membrane lesions
desmoglein
enzyme-linked immunosorbent assay
