摘要
目的 探讨survivin、p5 3、bcl 2蛋白在鼻NK /T淋巴瘤中的表达及其与细胞凋亡的关系。方法 应用TdT介导的dUTP缺口末端标记 (TUNEL)技术和免疫组织化学S P法 ,检测 2 4例鼻NK /T淋巴瘤和 17例良性淋巴结病变中细胞凋亡和survivin、p5 3、bcl 2蛋白的表达水平。 结果 survivin、p5 3、bcl 2蛋白在鼻NK /T淋巴瘤表达的阳性率分别为 45 .8% ( 11/2 4)、62 .5 % ( 15 /2 4)和 16.7% ( 4 /2 4)。survivin表达上调与 p5 3高表达密切相关 (P <0 .0 5 ) ,但与bcl 2蛋白表达无明显相关性 (P >0 .0 5 )。凋亡指数与survivin、p5 3蛋白表达明显相关 (P <0 .0 5 ) ,但与bcl 2蛋白表达无明显相关性 (P >0 .0 5 )。 结论 survivin基因的异常表达引起的凋亡抑制 ,可能在鼻NK/T淋巴瘤的发生进展中有一定的作用 ,且其与p5 3异常表达显著相关 ,但与bcl 2蛋白表达无明显相关性。鼻NK
Objective To explore the relationship of the expression of survivin,p53,bcl-2 gene with cell apoptosis in nasal NK/T cell lymphomas.Methods Tdt-mediatedUTP nick end labeling(TUNEL) and immunohistochemistry were used to study cell apoptosis and the expression of survivin,p53,bcl-2 genes in 24 nasal NK/T cell lymphoma samples and 17 benign lymph node lesions.Results The positive expression rate of survivin,p53,bcl-2 were 45.8%(11/24),62.5%(15/24) and 16.7%(4/24) respectively in nasal NK/T cell lymphoma samples.The increased expression of survivin gene closely correlated with the high expression of p53 gene(P<0.05),but had no relationship with bcl-2 expression(P>0.05).The apoptotic index significantly correlated with the expression of survivin and p53,but not with bcl-2 expression.ConclusionApoptosis inhibition caused by overexpression of survivin may be involved in the genesis and development of nasal NK/T cell lymphoma,and the expression of survivin has a close relationship with the abnormal expression of p53,but not with the expression of bcl-2.The regulation of apoptosis in nasal NK/T cell lymphoma is a complex process controled by many factors.
出处
《实用癌症杂志》
2003年第6期583-585,共3页
The Practical Journal of Cancer
基金
贵州省教委和贵州省卫生厅资助 (批准号 1 999 477)