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WTK1细胞在tk位点突变和DNA损伤与修复中的应用

Application of WTK1 Cells to tk Site Mutation and DNA Damage and Repair Capacity
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摘要 背景与目的:为WTK1细胞在遗传毒理学可同时应用于基因突变和DNA损伤的研究提供实验依据。材料与方法:分别用标准诱变剂甲基磺酸甲酯(MMS)和过氧化氢(H2O2)处理WTK1细胞,采用tk基因突变试验和单细胞凝胶电泳技术(single Cell GelElectrophoresis,SCGE)对细胞的tk位点突变和过氧化氢诱发的DNA损伤情况进行检测。结果:甲基磺酸甲酯可诱发WTK1细胞tk位点的突变,以诱发染色体畸变为主。过氧化氢诱发了WTK1细胞DNA的损伤,并有剂量反应关系。随着修复孵育时间的延长,彗星细胞尾长和彗星细胞出现率明显下降,与对照组比较,差异有显著性(P<0.01)。结论:WTK1细胞可同时应用于tk基因突变和DNA损伤与修复的研究。采用该细胞株可对化合物进行基因突变和DNA损伤进行研究评价。 BACKGROUND & AIM: To provide a basis in tk gene mutation and DNA damage and repair capacity research in WTK1 cells in toxicology field. MATERIAL AND METHODS: The tk site mutation frequency and DNA damage as well as repair capacity were detected after WTK1 cells treated by MMS and H2O2 respectively. RESULTS: MMS induced tk site mutation, the mutation colonies mainly were slow growth mutants (SG-mutant) that was chromosome aberration in aberration category. H2O2 could induce DNA damage in WTK1 cells and showed dose-response relationship. The quantity and the tail - length of Comet cells decrease significantly with incubation time. CONCLUSION: WTK1 cells could be a useful biology material used for testing tk gene mutation, DNA damage and repair capacity induced by chemicals.
出处 《癌变.畸变.突变》 CAS CSCD 2004年第1期1-4,共4页 Carcinogenesis,Teratogenesis & Mutagenesis
基金 国家自然科学基金资助项目(No.39770653)
关键词 WTK1细胞 tk位点 突变 DNA损伤 DNA修复 WTK1 cells tk site mutation DNA damage DNA repair capacity
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