摘要
目的研究90%单甲脒盐酸盐原药的致突变性。方法在活化和非活化条件下,采用小鼠淋巴瘤细胞株(L5178Y-3.7.2C)进行TK基因突变试验,分析不同剂量90%单甲脒盐酸盐原药(0.078～5.0 mg/ml)的致突变性。结果在有和无体外代谢活化系统S9时,1.25、0.625、0.313、0.156 mg/ml剂量组的90%单甲脒盐酸盐原药TK位点突变频率稍有增加,但与溶剂对照组比较,均无统计学意义(P>0.05)。结论在本体外哺乳动物细胞基因突变试验剂量范围内,未见90%单甲脒盐酸盐原药具有致突变性。
Objective To understand the mutagenicity of 90% N＇-（2,4-dimethylphenyl）-N＇-methylformamidine hydrochloride（DMAH）.Methods The L5178Y-3.7.2C cells were used for assessing the mutagenicity of 90%DMAH（0.078-5.0 mg/ml）,and the method of TK locus mutation assay was adopted under the activated and inactivated condition.Results Among 1.25 mg/ml,0.625 mg/ml,0.313 mg/ml and 0.156 mg/ml 90% DMAH groups,no significant differences were seen in the frequency of mutation in TK locus with or without metabolic activation system S9 compared with the solvent control group（P0.05）.Conclusion 90% DMAH is considered to be non-mutagenic to L5178Y-3.7.2C cells under the conditions of the test.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2011年第6期523-525,共3页
Journal of Environment and Health
关键词
90%单甲脒盐酸盐原药
TK基因
致突变
90% N＇-（2 4-dimethylphenyl）-N＇-methylformamidine hydrochloride TK gene Mutagenicity
